Hierarchical Fabrication of Engineered Vascularized Bone Biphasic Constructs via Dual 3D Bioprinting: Integrating Regional Bioactive Factors into Architectural Design

• Published in: Advanced healthcare materials Vol. 5; no. 17; pp. 2174 - 2181
• Main Authors: Cui, Haitao, Zhu, Wei, Nowicki, Margaret, Zhou, Xuan, Khademhosseini, Ali, Zhang, Lijie Grace
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.09.2016; Wiley Subscription Services, Inc
• Subjects: Biocompatibility; Bioengineering; Bone and Bones - blood supply; Bone and Bones - cytology; Bone and Bones - metabolism; Bones; Chemical reactions; click reaction; Design engineering; dual 3D bioprinting; Health care; Human Umbilical Vein Endothelial Cells - cytology; Human Umbilical Vein Endothelial Cells - metabolism; Humans; mussel-inspired chemistry; Platforms; Printing, Three-Dimensional; regional immobilization; Three dimensional printing; Tissue Engineering; vascularized complex tissue; dual 3D bioprinting; regional immobilization; vascularized complex tissue; click reaction; mussel-inspired chemistry
• ISSN: 2192-2640; 2192-2659; 2192-2659
• DOI: 10.1002/adhm.201600505

A biphasic artificial vascularized bone construct with regional bioactive factors is presented using dual 3D bioprinting platform technique, thereby forming a large functional bone grafts with organized vascular networks. Biocompatible mussel‐inspired chemistry and “thiol‐ene” click reaction are used to regionally immobilize bioactive factors during construct fabrication for modulating or improving cellular events.