Antibody response to Haemophilus influenzae outer membrane protein D, P6, and OMP26 after nasopharyngeal colonization and acute otitis media in children

• Published in: Vaccine Vol. 28; no. 44; pp. 7184 - 7192
• Main Authors: Pichichero, Michael E, Kaur, Ravinder, Casey, Janet R, Sabirov, Albert, Khan, M. Nadeem, Almudevar, Anthony
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 18.10.2010; Elsevier; Elsevier Limited
• Subjects: Acute otitis media; Adsorption; Age; Allergy and Immunology; Antibodies, Bacterial - blood; Antibody Formation; Applied microbiology; Bacterial infections; Bacterial Outer Membrane Proteins - immunology; Bacterial Proteins - immunology; Bacteriology; Biological and medical sciences; Candidates; Carriage; Carrier Proteins - immunology; Child, Preschool; Colleges & universities; Colonization; Ear diseases; Fundamental and applied biological sciences. Psychology; Haemophilus Infections - immunology; Haemophilus influenzae; Haemophilus influenzae - classification; Haemophilus influenzae - immunology; Haemophilus Vaccines - immunology; Humans; Immunoglobulin D - immunology; Immunoglobulin G - blood; Infant; Lipoproteins - immunology; Microbiology; Miscellaneous; Multilocus Sequence Typing; Nasopharynx - microbiology; Non-typeable Haemophilus influenzae; Otitis Media - immunology; Otitis Media - microbiology; P6 protein; Pathogenesis; Pediatrics; Prospective Studies; Protein D; Protein OMP26; Proteins; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Protein D; P6 protein; Acute otitis media; Non-typeable Haemophilus influenzae; Protein OMP26; Carriage; Human; Membrane protein; Immune response; Middle ear disease; Acute; External membrane; Haemophilus influenzae; Pasteurellaceae; Bacteria; ENT disease; Otitis media; Child; Humoral immunity
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2010.08.063

Abstract Development of natural antibodies to 3 nontypeable Haemophilus influenzae (NTHi) outer membrane proteins (D, P6 and OMP26) was prospectively studied in 130 children 6–30 months of age during NP colonization and acute otitis media (AOM). IgG antibody to protein D, P6 and OMP26 increased with age ( p < 0.001). Serum IgG responses to NP colonization were different for the 3 proteins: protein D responses occurred at a later age than P6, and OMP26 responses were minimal. For all 3 proteins serum antibody levels in the convalescent phase of AOM infection were not as high as after NP colonization. Antibodies to protein D and P6 but not OMP26 were bactericidal.