Determination of Acid Dissociation Constant of Pravastatin under Degraded Conditions by Capillary Zone Electrophoresis

• Published in: Analytical Sciences Vol. 31; no. 11; pp. 1193 - 1196
• Main Authors: TAKAYANAGI, Toshio, AMIYA, Mika, SHIMAKAMI, Natsumi, YABUTANI, Tomoki
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2015; Springer Nature Singapore; Nature Publishing Group
• Subjects: acid degradation; acid dissociation constant; Analytical Chemistry; Capillarity; Capillary zone; capillary zone electrophoresis; Chemistry; Constants; Degradation; Electrophoresis; Electrophoresis, Capillary - methods; Hydrogen-Ion Concentration; Mathematical analysis; micellar electrokinetic chromatography; Pravastatin; Pravastatin - chemistry; Time Factors; capillary zone electrophoresis; micellar electrokinetic chromatography; acid dissociation constant; Pravastatin; acid degradation
• ISSN: 0910-6340; 1348-2246; 1348-2246
• DOI: 10.2116/analsci.31.1193

The acid dissociation constant of pravastatin was determined under degraded conditions. Pravastatin was degraded in an acidic solution (pH = 2.0) for 5 h, and the degradation solution was subjected to the measurement of the effective electrophoretic mobility by capillary zone electrophoresis. Although the amount of pravastatin decreased by the acid degradation, its acid dissociation constant was successfully determined with the residual pravastatin through its effective electrophoretic mobility. The determined acid dissociation constant value agreed well with the one obtained with freshly prepared solution and with some reported values.