Development And Characterization Of Folic Acid Conjugated Multi Walled Carbon Nanotubes

The development of folic acid conjugated multi wall carbon nanotubes (FA-MWCNTs) allowed for the regulated and less toxic distribution of methotrecate (MTX). MWCNTs were functionalized one after the other to create MTX/FA-MWCNTs. In phosphate buffer solutions (PBS) with a pH of 7.4, MTX was physical...

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Bibliographic Details
Published inFrontiers in health informatics pp. 6606 - 6615
Main Authors Kayat, Jitendra, Tiwari, Pooja, Chundawat, Rituraj Singh, Sharma, Neeraj, Jain, Ruchi
Format Journal Article
LanguageEnglish
Published 25.06.2025
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Summary:The development of folic acid conjugated multi wall carbon nanotubes (FA-MWCNTs) allowed for the regulated and less toxic distribution of methotrecate (MTX). MWCNTs were functionalized one after the other to create MTX/FA-MWCNTs. In phosphate buffer solutions (PBS) with a pH of 7.4, MTX was physically loaded onto virgin MWCNTs and FA-MWCNTs. The entrapment efficiency, in vitro release, hemolytic toxicity, and ex vivo tests on the lung epithelial cancer cell line "A-549" were assessed. MTX was effectively incorporated into both the pristine and FA-MWCNT formulations, with the FA-MWCNTS formulation exhibiting the maximum trapping of MTX at 90.3 ± 1.8%. The study examined the in-vitro release of MTX from MTX/FA-MWCNTs at 7.4 (PBS), exhibiting a quicker initial release followed by a sustained release. Furthermore, it was discovered that, when applied to the lung epithelial cancer cell line "A-549," MTX/FA-MWCNTs was more cytotoxic and less hemolytic than free MTX.
ISSN:2676-7104
2676-7104
DOI:10.63682/fhi1737