Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis

• Published in: Dental Materials Journal Vol. 34; no. 2; pp. 154 - 160
• Main Authors: MALKOÇ, Meral ARSLAN, DEMİR, Necla, ŞENGÜN, Abdulkadir, BOZKURT, Şerife Buket, HAKKI, Sema Sezen
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japanese Society for Dental Materials and Devices 2015; Japanese Society for Dental Materials and Devices
• Subjects: Analysis of variance; Animals; Cattle; Cells, Cultured; Cements; Cytotoxicity; Dental materials; Dental Pulp - cytology; Dental Pulp - drug effects; Dental pulp-derived cells; Dentistry; Materials selection; Materials Testing; Polymers; Real time; Real-time cell analysis; Resin cements; Resin Cements - adverse effects; Resin Cements - pharmacology; Resins; Survival
• ISSN: 0287-4547; 1881-1361
• DOI: 10.4012/dmj.2014-167

To evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs), Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials were incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival. The statistical analyses used were one-way ANOVA and Tukey-Kramer tests. BQM, RU200, and ME demonstrated a significant decrease in the bDPCs’ index at 24 and 72 h (p≤0.001). These materials were found to be the most toxic resin cements, as compared to the control and other tested materials (C2 and MA). However, C2 and MA showed a better survival rate, compared to BQM, RU200, and ME, and had lower cell index than the control group. The cytotoxic effects of resin cements on pulpa should be evaluated during the selection of proper cements.