Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis

• Published in: Journal of extracellular vesicles Vol. 2; no. 1; pp. 1 - n/a
• Main Authors: Gardiner, Chris, Ferreira, Yannick J., Dragovic, Rebecca A., Redman, Christopher W.G., Sargent, Ian L.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.01.2013; John Wiley & Sons, Inc; Co-Action Publishing; Wiley
• Subjects: Atoms & subatomic particles; Calibration; Cameras; Colleges & universities; Enumeration; Extracellular vesicles; Flow cytometry; Full text; Laboratories; Lasers; light scattering; Methods; Microspheres; nanoparticle Tracking Analysis; Nanoparticles; Obstetrics; Original; Particle size; Software; standardisation; Vibration; Viscosity; United Kingdom > UK; Japan; standardisation; light scattering; extracellular vesicles; nanoparticle tracking analysis
• ISSN: 2001-3078; 2001-3078
• DOI: 10.3402/jev.v2i0.19671

Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique. Access the supplementary material to this article: Refractive index estimation (see Supplementary files under Article Tools online).