Effects of Rabeprazole, a Gastric Proton Pump Inhibitor, on Biliary and Hepatic Lysosomal Enzymes in Rats

• Published in: Japanese Journal of Pharmacology Vol. 76; no. 3; pp. 279 - 288
• Main Authors: Fujisaki, Hideaki, Oketani, Kiyoshi, Nagakawa, Jun-ichi, Takenaka, Osamu, Yamanishi, Yoshiharu
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japanese Pharmacological Society 1998
• Subjects: 2-Pyridinylmethylsulfinylbenzimidazoles; Acetylglucosaminidase - metabolism; Animals; Benzimidazoles - pharmacology; beta-Galactosidase - metabolism; Bile - drug effects; Bile - enzymology; Biliary secretion; Chloroquine; Chloroquine - pharmacology; Enzyme Inhibitors - pharmacology; Gastric Mucosa - drug effects; Gastric Mucosa - metabolism; H+, K+-ATPase inhibitor; K+-ATPase inhibitor; Lipids - blood; Liver - drug effects; Liver - enzymology; Lysosomal enzyme; Lysosomes - drug effects; Lysosomes - enzymology; Male; Omeprazole - pharmacology; Proton Pump Inhibitors; Rabeprazole; Rats; Rats, Sprague-Dawley; H; Lysosomal enzyme; Rabeprazole; K+-ATPase inhibitor; Chloroquine; Biliary secretion
• ISSN: 0021-5198; 1347-3506
• DOI: 10.1254/jjp.76.279

The effects of rabeprazole (E3810), omeprazole and chloroquine on hepatic lysosomal function were studied. After chloroquine (50 mg/kg), rabeprazole (5 mg/kg) or omeprazole (5 mg/kg) was given intraperitoneally to rats for 6 days, the bile was collected via a bile duct cannula for 5 hr, and hepatic and biliary lysosomal enzyme (N-acetyl-β-glucosaminidase and β-galactosidase) activities were measured. The latency (an index for the hepatic lysosomal membrane integrity) was calculated from the N-acetyl-β-glucosaminidase activity. The biliary constituents and plasma concentrations of lipids were also measured. The administration of chloroquine significantly increased hepatic and biliary lysosomal enzyme activities, but did not affect the lysosomal enzyme latency, hepatic and biliary protein content or bile flow. It significantly decreased the bile acid level. On the other hand, the administration of rabeprazole and omeprazole did not alter the lysosomal enzyme activities, lysosomal enzyme latency, protein content in liver or liver weight. Furthermore, no significant differences were observed in biliary lysosomal enzyme activity, protein content, bile flow, biliary constituents or in the plasma concentrations of lipids between the drug groups (rabeprazole or omeprazole) and the control group. The results of the present study indicate that rabeprazole, like omeprazole, does not influence hepatic lysosomal function.