Hesperidin ameliorates H2O2-induced bovine mammary epithelial cell oxidative stress via the Nrf2 signaling pathway

• Published in: Journal of animal science and biotechnology Vol. 15; no. 1; p. 57
• Main Authors: Huang, Qi, Liu, Jiashuo, Peng, Can, Han, Xuefeng, Tan, Zhiliang
• Format: Journal Article
• Language: English
• Published: London BioMed Central 09.04.2024; CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Hunan Provincial Key Laboratory of Ani-mal Nutritional Physiology and Metabolic Process,Institute of Subtropical Agri-culture,Chinese Academy of Sciences,Changsha 410125,Hunan,China; State Key Laboratory of Animal Nutrition and Feeding,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China%CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Hunan Provincial Key Laboratory of Ani-mal Nutritional Physiology and Metabolic Process,Institute of Subtropical Agri-culture,Chinese Academy of Sciences,Changsha 410125,Hunan,China; BMC
• Subjects: antioxidant activity; Antioxidants; Apoptosis; Bovine mammary epithelial cell; Catalase; Cattle; Cell proliferation; Cells; Citrus; Dairy cattle; drugs; Epithelial cells; Epithelium; Feed additives; Flavonoids; Food additives; Free radicals; Gene expression; Hesperidin; Hydrogen peroxide; Inflammation; malondialdehyde; Mammary gland; Membrane potential; mitochondrial membrane; Molecular modelling; Nrf2 signaling pathway; Nuclear transport; Oxidative stress; Proteins; Reactive oxygen species; Scavenging; Signal transduction; Translocation; St Louis Missouri; United States > US; China; Oxidative stress; Nrf2 signaling pathway; Hesperidin; Bovine mammary epithelial cell
• ISSN: 2049-1891; 1674-9782; 2049-1891
• DOI: 10.1186/s40104-024-01012-9

BackgroundHesperidin is a citrus flavonoid with anti-inflammatory and antioxidant potential. However, its protective effects on bovine mammary epithelial cells (bMECs) exposed to oxidative stress have not been elucidated.ResultsIn this study, we investigated the effects of hesperidin on H2O2-induced oxidative stress in bMECs and the underlying molecular mechanism. We found that hesperidin attenuated H2O2-induced cell damage by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels, increasing catalase (CAT) activity, and improving cell proliferation and mitochondrial membrane potential. Moreover, hesperidin activated the Keap1/Nrf2/ARE signaling pathway by inducing the nuclear translocation of Nrf2 and the expression of its downstream genes NQO1 and HO-1, which are antioxidant enzymes involved in ROS scavenging and cellular redox balance. The protective effects of hesperidin were blocked by the Nrf2 inhibitor ML385, indicating that they were Nrf2 dependent.ConclusionsOur results suggest that hesperidin could protect bMECs from oxidative stress injury by activating the Nrf2 signaling pathway, suggesting that hesperidin as a natural antioxidant has positive potential as a feed additive or plant drug to promote the health benefits of bovine mammary.