MONOCLONAL ANTIBODY RAISED AGAINST APOPROTEIN OF C-1027: EFFECT ON BIOCHEMICAL AND BIOLOGICAL ACTIVITIES OF THE HOLOANTIBIOTIC

• Published in: Journal of antibiotics Vol. 47; no. 7; pp. 787 - 791
• Main Authors: TSUCHIYA, KAYOKO S., ARITA, MICHITSUNE, HORI, MAKOTO, OTANI, TOSMO
• Format: Journal Article
• Language: English
• Published: Tokyo JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 1994; Japan Antibiotics Research Association
• Subjects: Aminoglycosides; Animals; Anti-Bacterial Agents - antagonists & inhibitors; Anti-Bacterial Agents - immunology; Anti-Bacterial Agents - pharmacology; Antibiotics, Antineoplastic - antagonists & inhibitors; Antibiotics, Antineoplastic - immunology; Antibiotics, Antineoplastic - pharmacology; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacology; Antibody Specificity; Antineoplastic agents; Apoproteins - antagonists & inhibitors; Apoproteins - immunology; Apoproteins - pharmacology; Biological and medical sciences; Carcinoma, Ehrlich Tumor - drug therapy; Drug Interactions; Enediynes; General aspects; Immunoglobulin G - pharmacology; Medical sciences; Peptides; Pharmacology. Drug treatments; Antineoplastic agent; Antibiotic; Apoproteins; Targeted drug; Cytotoxicity; Monoclonal antibody; Target cell; In vitro; Biological activity; Tumor cell
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.47.787

A monoclonal antibody raised against C-1027 apoprotein recognized not only the apoprotein, but also the holoantibiotic (Antibiotic C-1027) almost equally. Among the biochemical and biological activities of the holoantibiotic, the antibody inhibited the aminopeptidase activity and the cyto-toxicity to Ehrlich carcinoma cells in cultures, but not the DNA-cleaving activity in vitro. The immunohistogram, using this antibody, of Ehrlich carcinoma cells that had been exposed to the holoantibiotic suggested penetration of the holoantibiotic into target cells.