Postoperative Changes in Aqueous Monocyte Chemotactic Protein-1 Levels and Bleb Morphology after Trabeculectomy vs. Ex-PRESS Shunt Surgery

• Published in: PloS one Vol. 10; no. 10; p. e0139751
• Main Authors: Shobayashi, Kohei, Inoue, Toshihiro, Kawai, Motofumi, Iwao, Keiichiro, Ohira, Saori, Kojima, Sachi, Kuroda, Utako, Nakashima, Kei-Ichi, Tanihara, Hidenobu
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.10.2015; Public Library of Science (PLoS)
• Subjects: Animals; Anterior Eye Segment - metabolism; Anterior Eye Segment - pathology; Anterior Eye Segment - surgery; Biomarkers - metabolism; Blister - metabolism; Blister - pathology; Blister - surgery; Chemokine CCL2 - metabolism; Enzyme-Linked Immunosorbent Assay; Female; Glaucoma; Glaucoma - metabolism; Glaucoma - pathology; Glaucoma - surgery; Glaucoma Drainage Implants; Health aspects; Image Processing, Computer-Assisted; Immunoenzyme Techniques; Intraocular pressure; Life assessment; Life sciences; Medical imaging; Medical research; Monocyte chemoattractant protein 1; Monocytes; Morphology; Ophthalmologic Surgical Procedures; Optical Coherence Tomography; Ostomy; Physiological aspects; Postoperative Period; Proteins; Rabbits; Surgery; Tomography; Trabeculectomy; Trabeculectomy - methods; Wound healing; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0139751

To evaluate the postoperative changes in blebs and levels of aqueous monocyte chemotactic protein-1 (MCP-1) after trabeculectomy vs. Ex-PRESS tube shunt surgery. Rabbits were subjected to trabeculectomy or Ex-PRESS tube shunt surgery and observed for up to 3 months. Intraocular pressure (IOP) was measured using a rebound tonometer. The MCP-1 level was measured by enzyme-linked immunosorbent assay (ELISA). Bleb morphology was evaluated using photos and anterior-segment optical coherence tomography (OCT). There were no differences in bleb appearance or IOP at any time between the groups. Bleb wall density in the anterior-segment OCT image was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group. The MCP-1 level in control eyes was 304.1 ± 45.2 pg/mL. In the trabeculectomy group, the mean aqueous MCP-1 level was 1444.9, 1914.3, 1899.8, 516.4, 398.3, 427.3, 609.5, 1612.7, 386.2, and 167.9 pg/mL at 3, 6, and 12 h, and 1, 2, 5, 7, 14, 30, and 90 days after surgery, respectively. In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL. There were no significant differences in the aqueous MCP-1 level between the groups at any time point. The postoperative changes were similar in the Ex-PRESS and trabeculectomy groups, except for bleb wall density in the anterior-segment OCT image. The postoperative aqueous MCP-1 level had bimodal peaks in both groups.