Fibroblast Migration in 3D is Controlled by Haptotaxis in a Non-muscle Myosin II-Dependent Manner

• Published in: Annals of biomedical engineering Vol. 43; no. 12; pp. 3025 - 3039
• Main Authors: Moreno-Arotzena, O., Borau, C., Movilla, N., Vicente-Manzanares, M., García-Aznar, J. M.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2015; Springer Nature B.V
• Subjects: Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Biomimetics; Biophysics; Cell Movement - drug effects; Cell Movement - physiology; Cells, Cultured; Classical Mechanics; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - physiology; Human; Humans; Hydrogels; Microfluidics; Migration; Myosin; Myosin Type II - physiology; Platforms; Proto-Oncogene Proteins c-sis - pharmacology; Three dimensional; Three dimensional models; Mechanical properties; Fibrin; Hydrogel; Chemotaxis; Collagen; Microfluidics
• ISSN: 0090-6964; 1573-9686
• DOI: 10.1007/s10439-015-1343-2

Cell migration in 3D is a key process in many physiological and pathological processes. Although valuable knowledge has been accumulated through analysis of various 2D models, some of these insights are not directly applicable to migration in 3D. In this study, we have confined biomimetic hydrogels within microfluidic platforms in the presence of a chemoattractant (platelet-derived growth factor-BB). We have characterized the migratory responses of human fibroblasts within them, particularly focusing on the role of non-muscle myosin II. Our results indicate a prominent role for myosin II in the integration of chemotactic and haptotactic migratory responses of fibroblasts in 3D confined environments.