Quantification of heat shock proteins in the posterior interosseous nerve among subjects with type 1 and type 2 diabetes compared to healthy controls

• Published in: Frontiers in neuroscience Vol. 17; p. 1227557
• Main Authors: Ising, Erik, Åhrman, Emma, Thomsen, Niels O. B., Åkesson, Anna, Malmström, Johan, Dahlin, Lars B.
• Format: Journal Article
• Language: English
• Published: Lausanne Frontiers Research Foundation 2023; Frontiers Media S.A
• Subjects: Anesthesia; Biopsy; Carpal tunnel syndrome; Clinical Medicine; Diabetes; Diabetes mellitus (insulin dependent); Diabetes mellitus (non-insulin dependent); Electrophysiology; Endocrinology and Diabetes; Endokrinologi och diabetes; Heat shock proteins; Hypoglycemia; Ions; Kinases; Klinisk medicin; Mass spectrometry; Mass spectroscopy; Medical and Health Sciences; Medical screening; Medicin och hälsovetenskap; Morphometry; neuropathy; Neuroscience; Orthopaedics; Orthopedics; Ortopedi; Oxidative stress; Peripheral nerves; Peripheral neuropathy; Proteomics; Scientific imaging; Statistical analysis; type 1 diabetes; type 2 diabetes; Velocity; neuropathy; type 1 diabetes; type 2 diabetes; proteomics; heat shock proteins; diabetes
• ISSN: 1662-453X; 1662-4548; 1662-453X
• DOI: 10.3389/fnins.2023.1227557

Introduction: Diabetic peripheral neuropathy (DPN) is a common complication to both type 1 (T1D) and type 2 diabetes (T2D). No cure for DPN is available, but several potential targets have been proposed for treatment. Heat shock proteins (HSPs) are known to respond to both hyper-and hypoglycaemia. DPN can be diagnosed using electrophysiology and studied using peripheral nerve biopsies.To study presence and patterns of HSPs in peripheral nerve biopsies from subjects with T1D, T2D and healthy controls.Methods: Posterior interosseous nerves (PIN) from a total of 56 subjects with T1D (n = 9), T2D (n = 24) and without diabetes (i.e., healthy controls, n = 23) were harvested under local anaesthesia and prepared for quantitative mass spectrometry analysis. Protein intensities were associated to electrophysiology data of ulnar nerve and morphometry of the same PIN, and differences in protein intensities between groups were analysed.In total, 32 different HSPs were identified and quantified in the nerve specimens. No statistically significant differences were seen regarding protein intensities between groups. Furthermore, protein intensities did not correlate to amplitude or conduction velocity in ulnar nerve or with myelinated nerve fibre density of PIN.Quantitative proteomics can be used to study HSPs in nerve biopsies, but no clear differences in protein quantities were seen between groups in this cohort.