Role of pore size location in determining bacterial activity during predation by protozoa in soil

• Published in: Applied and Environmental Microbiology Vol. 61; no. 10; pp. 3537 - 3543
• Main Authors: Wright, D.A. (University of Teesside, Middlesborogh, Cleveland, UK.), Killham, K, Glover, L.A, Prosser, J.I
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.10.1995
• Subjects: Animal, plant and microbial ecology; Bacteria; Bacteriology; Biological and medical sciences; CILIOPHORA; Colpoda steinii; DEPREDACION; DINAMICA DE LA POBLACION; DYNAMIQUE DES POPULATIONS; ESPECE; ESPECIES; FLORA DEL SUELO; FLORE DU SOL; Fundamental and applied biological sciences. Psychology; GENE; GENES; Microbial ecology; OXIDORREDUCTASAS; OXYDOREDUCTASE; POROSITE DU SOL; PREDATION; PROPIEDADES OPTICAS; PROPRIETE OPTIQUE; PSEUDOMONAS FLUORESCENS; RELACIONES PREDATOR PRESA; RELATION PREDATEUR PROIE; SISTEMA POROSO DEL SUELO; Soil; Soils; SUPERVIVENCIA; SURVIE; VARIEDADES NATURALIZADAS; VARIETE INTRODUITE; Pseudomonadales; Protozoa; Microbial activity; Soil structure; Pseudomonas fluorescens; Microbial biomass; Pore size; Spatial distribution; Bacteria; Pseudomonadaceae; Nutrient; Ciliata; Population dynamics; Predation; Sandy loam soil
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.61.10.3537-3543.1995

The predation of a luminescence-marked strain of Pseudomonas fluorescens by the soil ciliate Colpoda steinii was studied in soil microcosms. Bacterial cells were introduced in either small (neck diameter, 6 micrometer) or intermediate sized (neck diameter, 6 to 30 micrometer) pores in the soil by inoculation at appropriate matric potentials, and ciliates were introduced into large pores (neck diameter, 30 to 60 micrometer). Viable cell concentrations of bacteria introduced into intermediate-sized pores decreased at a greater rate than those in small pores, with reductions in bacterial populations being accompanied by an increase in viable cell numbers of the ciliate. The data indicate that the location of bacteria in small pores provides significant protection from predation. In the absence of C. steinii, the level of metabolic activity of the bacterial population, measured by luminometry, decreased at a greater rate than cell number, and the level of luminescence per cell consequently decreased. The decrease in levels of luminescence indicates a loss of activity due to starvation. During predation by C. steinii, the level of the activity of cells introduced into small pores fell in a similar manner. The level of cell activity was, however, significantly greater for cells introduced into intermediate-sized pores, despite their greater susceptibility to predation. The data suggest that increased activity arises from a release of nutrients by the predator and the greater accessibility of bacteria to nutrients in larger pores. Nutrient amendment of microcosms resulted in increases in bacterial populations to sustained, higher levels, while levels of luminescence increased transiently. The predation of cells introduced into intermediate-sized pores was greater, and there was also evidence that the level of activity of surviving bacteria was greater for bacteria in intermediate-sized but not small pores