Application of Genomic In Situ Hybridization in Horticultural Science

Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualizati...

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Bibliographic Details
Published inInternational journal of genomics Vol. 2017; no. 2017; pp. 1 - 12
Main Authors Ramzan, Fahad, Lim, K.-B., Younis, Adnan
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2017
Hindawi
Hindawi Limited
Subjects
Chromosomes
Cytogenetics
Deoxyribonucleic acid
Discrimination
DNA
Evolution
Flowers & plants
Genomes
Horticulture
Hybridization
Identification
Restitution
Review
Rhododendron
Science
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Summary:Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools to analyze the genomic structure and function, chromosome constituents, recombination patterns, alien gene introgression, genome evolution, aneuploidy, and polyploidy and also genome constitution visualization and chromosome discrimination from different genomes in allopolyploids of various horticultural crops. Using GISH advancement as multicolor detection is a significant approach to analyze the small and numerous chromosomes in fruit species, for example, Diospyros hybrids. This analytical technique has proved to be the most exact and effective way for hybrid status confirmation and helps remarkably to distinguish donor parental genomes in hybrids such as Clivia, Rhododendron, and Lycoris ornamental hybrids. The genome characterization facilitates in hybrid selection having potential desirable characteristics during the early hybridization breeding, as this technique expedites to detect introgressed sequence chromosomes. This review study epitomizes applications and advancements of genomic in situ hybridization (GISH) techniques in horticultural plants.
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Academic Editor: Graziano Pesole
ISSN:2314-436X
2314-4378
DOI:10.1155/2017/7561909