Effects of newly-developed retrograde filling material on osteoblastic differentiation in vitro

• Published in: Dental Materials Journal Vol. 38; no. 4; pp. 528 - 533
• Main Authors: LEE, Go-Woon, YOON, Ji-Hye, JANG, Ji-Hyun, CHANG, Hoon-Sang, HWANG, Yun-Chan, HWANG, In-Nam, OH, Won-Mann, LEE, Bin-Na
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japanese Society for Dental Materials and Devices 26.07.2019; Japanese Society for Dental Materials and Devices; Japan Science and Technology Agency
• Subjects: Alkaline phosphatase; Biomedical materials; Cell viability; Chemical properties; Crystals; Dentistry; Differentiation; Endosequence; Fillers; Gene expression; MTA; Organic chemistry; Osteoblastic differentiation; Osteoblastogenesis; Osteoblasts; pH effects; Scanning electron microscopy; Staining; Osteoblastic differentiation; Cell viability; MTA; Endosequence
• ISSN: 0287-4547; 1881-1361
• DOI: 10.4012/dmj.2018-124

The aim of this study was to compare chemical properties and bioactivities of with mineral trioxide aggregate (MTA) and EndoSequence Root Repair Material (ERRM). After setting, surfaces of test materials were observed by scanning electron microscope (SEM). The pH and cell viability of materials were tested. Osteoblastic differentiation and alkaline phosphatase (ALP) activity were measured by quantitative real-time PCR and ALP staining. MTA showed spindle-shaped crystals while ERRM showed round-shaped crystals of various sizes. ERRM presented lower pH than MTA. Both materials showed good cell viabilities compared to the control. Expression levels of osteoblastic genes and ALP staining were increased significantly (p<0.05) in ERRM and MTA groups compared to those in the control group. In conclusion, ERRM and MTA both had effects on osteoblastic differentiation. Therefore, ERRM can be used as a desirable alternative to MTA for root-end filling material.