Molecular and Functional Analysis of Cellular Phenomena Using Single-Cell Mass Spectrometry
Single-cell analysis has attracted attention in many fields of biological studies as a tool to survey the precise mechanisms of cellular and molecular behavior. The development of sensitive mass spectrometry allows the study of molecules in single cells or small regions. Matrix-assisted laser desorp...
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Published in | Biological & pharmaceutical bulletin Vol. 35; no. 9; pp. 1425 - 1431 |
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Main Authors | , , |
Format | Journal Article |
Language | English Japanese |
Published |
Japan
The Pharmaceutical Society of Japan
2012
Pharmaceutical Society of Japan Japan Science and Technology Agency |
Subjects | |
Online Access | Get full text |
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Summary: | Single-cell analysis has attracted attention in many fields of biological studies as a tool to survey the precise mechanisms of cellular and molecular behavior. The development of sensitive mass spectrometry allows the study of molecules in single cells or small regions. Matrix-assisted laser desorption/ionization imaging mass spectrometry and secondary-ion mass spectrometry use in situ ionization of specimens on sample plates to visualize molecular distributions as images from mass spectra. Several single-cell mass spectrometry technologies that initially recover a single cell followed by ionization have been developed. Among them, only nanospray-mediated sampling and ionization named Live Single-cell Mass Spectrometry can be used for real-time analysis. This paper explains that method in detail. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 0918-6158 1347-5215 |
DOI: | 10.1248/bpb.b212012 |