Induction of CD4$^+$ Human Cytolytic T Cells Specific for HIV-Infected Cells by a gp160 Subunit Vaccine

• Published in: Science (American Association for the Advancement of Science) Vol. 248; no. 4960; pp. 1234 - 1237
• Main Authors: Orentas, Rimas J., James E. K. Hildreth, Obah, Eugene, Polydefkis, Michael, Smith, Gale E., Clements, Mary Lou, Siliciano, Robert F.
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 08.06.1990; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Antigens; Biological and medical sciences; Cells, Cultured; Clone Cells; Cultured cells; Cytotoxicity, Immunologic; env genes; Fundamental and applied biological sciences. Psychology; Gene Products, env - immunology; Genetic vectors; HIV; HIV - immunology; HIV Envelope Protein gp160; HIV infection; HIV infections; HIV Seropositivity; Humans; Immunization; Infections; Lymphocytes; Macromolecular Substances; Medical research; Microbiology; Prevention; Protein Precursors - immunology; Proteins; Recombinant Proteins - immunology; T cells; T lymphocytes; T-Lymphocytes, Cytotoxic - immunology; Vaccination; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccinia; Viral Vaccines - immunology; Virology; Viruses; Volunteerism; Human; Cytolysis; HIV-1 virus; Cytotoxicity test; Retroviridae; Cytotoxicity; Glycoproteins; Cellular immunity; Vaccine; In vitro; Virus; T-Lymphocyte; Lentivirinae; Human immunodeficiency virus; Infected cell; Virus envelope
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.2190315

Cytolytic T lymphocyte (CTL) responses were evaluated in humans immunized with recombinant human immunodeficiency virus type 1 (HIV) envelope glycoprotein gp160. Some vaccinees had gp160-specific CTLs that were shown by cloning to be CD4$^+$. Although induced by exogenous antigen, most gp160-specific CTL clones also recognized gp160 synthesized endogenously in target cells. These clones lysed autologous CD4$^+$ T lymphoblasts infected with HIV. Of particular interest were certain vaccine-induced clones that lysed HIV-infected cells, recognized gp160 from diverse HIV isolates, and did not participate in "innocent bystander" killing of noninfected CD4$^+$ T cells that had bound gp120.