Production and characterization of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of Japanese bunching onion (A. fistulosum L.)

• Published in: Genes & Genetic Systems Vol. 79; no. 5; pp. 263 - 269
• Main Authors: Hang, T.T.M. (Tottori Univ. (Japan)), Shigyo, M, Yamauchi, N, Tashiro, T
• Format: Journal Article
• Language: English
• Published: Japan The Genetics Society of Japan 01.10.2004; Japan Science and Technology Agency
• Subjects: alien addition line; Allium - enzymology; Allium - genetics; ALLIUM CEPA; ALLIUM FISTULOSUM; Aneuploidy; Aspartate Aminotransferase, Mitochondrial - analysis; BACKCROSSING; CHROMOSOMES; Chromosomes, Plant - physiology; Crosses, Genetic; Flow Cytometry; INTERSPECIFIC HYBRIDIZATION; ISOENZYMES; Isoenzymes - analysis; isozyme; Onions - genetics; POLYPLOIDY; PROGENY
• ISSN: 1341-7568; 1880-5779
• DOI: 10.1266/ggs.79.263

First and second backcrosses of amphidiploid hybrids (2n = 4x =32, genomes AAFF) between shallot (Allium cepa Aggregatum group) and A. fistulosum were conducted to produce A. cepa -A. fistulosum alien addition lines. When shallot (A. cepa Aggregatum group) was used as a pollinator, the amphidiploids and allotriploids set germinable BC sub(1) and BC sub(2) seeds, respectively. The 237 BC sub(1) plants mainly consisted of 170 allotriploids (2n = 3x = 24, AAF) and 42 hypo-allotriploids possessing 23 chromosomes, i.e., single-alien deletions (2n = 3x-1 = 23, AAFnF). The single-alien deletions in the BC sub(1) progeny showed dwarfing characteristics and were discriminated from the allotriploids (2n = 24) and hyper-allotriploids (2n = 25) by means of flow cytometric analysis. The chromosome numbers of 46 BC sub(2) seedlings varied from 16 to 24. Eight monosomic additions (2n = 2x+1 = 17, AA+nF) and 20 single-alien deletions were found in these BC sub(2) seedlings. Consequently, six kinds of A. cepa -A. fistulosum alien chromosome additions possessing different chromosome numbers (2n = 17, 18, 20, 21, 22, 23) were recognized in the BC sub(1) and BC sub(2) populations. A total of 79 aneuploids, including 62 single-alien deletions, were analyzed by a chromosome 6F-specific isozyme marker (Got-2) in order to recognize its existence in their chromosome complements. This analysis revealed that two out of 62 single-alien deletions did not possess 6F. One (AAF6F) out of the possible eight single-alien deletions could be identified at first. The present study is a first step toward the development of a useful tool, such as a complete set of eight different single-alien deletions, for the rapid chromosomal assignment of genes and genetic markers in A. fistulosum.