Genome sequence of the recombinant protein production host Pichia pastoris
Pichia pastoris has been a workhorse of protein production for decades. De Schutter et al . present its genomic sequence, which will allow development of improved strains. The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying pero...
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Published in | Nature biotechnology Vol. 27; no. 6; pp. 561 - 566 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Nature Publishing Group US
01.06.2009
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Pichia pastoris
has been a workhorse of protein production for decades. De Schutter
et al
. present its genomic sequence, which will allow development of improved strains.
The methylotrophic yeast
Pichia pastoris
is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation.
P. pastoris
strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for
P. pastoris
. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of
P. pastoris
. We also provide manually curated annotation for its 5,313 protein-coding genes. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1087-0156 1546-1696 |
DOI: | 10.1038/nbt.1544 |