Genome sequence of the recombinant protein production host Pichia pastoris

• Published in: Nature biotechnology Vol. 27; no. 6; pp. 561 - 566
• Main Authors: Van de Peer, Yves, Lin, Yao-Cheng, Glinka, Sascha, Rouzé, Pierre, De Schutter, Kristof, Tiels, Petra, Callewaert, Nico, Weber-Lehmann, Jacqueline, Van Hecke, Annelies
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2009; Nature Publishing Group
• Subjects: Agriculture; Base Sequence; Bioinformatics; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Cellular biology; Codon - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Genes, Fungal; Genetic aspects; Genetics; Genome, Fungal; Genomics; Glycosylation; Life Sciences; Metabolic Networks and Pathways - genetics; Methanol; Mission oriented research; Molecular Sequence Data; Phylogeny; Physiological aspects; Pichia - genetics; Pichia pastoris; Proteins; Recombinant Proteins; RNA, Transfer - genetics; Sequence Analysis, DNA; Yeast; Yeast fungi; Yeasts; Belgium; Germany; Ascomycota; Fungi; Gene; Production; Pichia pastoris; Recombinant protein; Genome; Sequencing; Annotation
• ISSN: 1087-0156; 1546-1696
• DOI: 10.1038/nbt.1544

Pichia pastoris has been a workhorse of protein production for decades. De Schutter et al . present its genomic sequence, which will allow development of improved strains. The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as a model organism for studying peroxisomal biogenesis and methanol assimilation. P. pastoris strains capable of human-type N-glycosylation are now available, which increases the utility of this organism for biopharmaceutical production. Despite its biotechnological importance, relatively few genetic tools or engineered strains have been generated for P. pastoris . To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris . We also provide manually curated annotation for its 5,313 protein-coding genes.