N-linked sugar chain of 55-kDa royal jelly glycoprotein
An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507-509 (1995)], has been identified. The sugar chains were...
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Published in | Bioscience, biotechnology, and biochemistry Vol. 60; no. 12; pp. 2099 - 2102 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Tokyo
Taylor & Francis
01.12.1996
Japan Society for Bioscience Biotechnology and Agrochemistry Oxford University Press |
Subjects | |
Online Access | Get full text |
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Summary: | An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507-509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz
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H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1→2Manα1→6(Manα1→2Manα1→3)Manαl→6(Manα1→2Manα1→2Manα1 →3)Manβ1→4GlcNAcβl→4GlcNAc, which is a non-processed high mannose type structure. |
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Bibliography: | Q04 1998003635 L51 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0916-8451 1347-6947 |
DOI: | 10.1271/bbb.60.2099 |