miR-1285-3p targets TPI1 to regulate the glycolysis metabolism signaling pathway of Tibetan sheep Sertoli cells

• Published in: PloS one Vol. 17; no. 9; p. e0270364
• Main Authors: An, Xuejiao, Li, Taotao, Chen, Nana, Wang, Huihui, Su, Manchun, Shi, Huibin, Duan, Xinming, Ma, Youji
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 22.09.2022; Public Library of Science (PLoS)
• Subjects: 3' Untranslated regions; Animals; Apoptosis; Biology and Life Sciences; Energy resources; Enzymes; Genes; Genetic aspects; Glycolysis; Health aspects; Lactic acid; Metabolic pathways; Metabolism; Methods; MicroRNA; MicroRNAs; Mutation; People and Places; Proteins; Research and Analysis Methods; Sertoli cells; Sheep; Signal transduction; Signaling; Sperm; Spermatogenesis; Stem cells; Substrates; China; Gansu China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0270364

Glycolysis in Sertoli cells (SCs) can provide energy substrates for the development of spermatogenic cells. Triose phosphate isomerase 1 (TPI1) is one of the key catalytic enzymes involved in glycolysis. However, the biological function of TPI1 in SCs and its role in glycolytic metabolic pathways are poorly understood. On the basis of a previous research, we isolated primary SCs from Tibetan sheep, and overexpressed TPI1 gene to determine its effect on the proliferation, glycolysis, and apoptosis of SCs. Secondly, we investigated the relationship between TPI1 and miR-1285-3p, and whether miR-1285-3p regulates the proliferation and apoptosis of SCs, and participates in glycolysis by targeting TPI1. Results showed that overexpression of TPI1 increased the proliferation rate and decreased apoptosis of SCs. In addition, overexpression of TPI1 altered glycolysis and metabolism signaling pathways and significantly increased amount of the final product lactic acid. Further analysis showed that miR-1285-3p inhibited TPI1 by directly targeting its 3'untranslated region. Overexpression of miR-1285-3p suppressed the proliferation of SCs, and this effect was partially reversed by restoration of TPI1 expression. In summary, this study shows that the miR-1285-3p/TPI1 axis regulates glycolysis in SCs. These findings add to our understanding on the regulation of spermatogenesis in sheep and other mammals.