Impact of serum and follicular fluid kisspeptin and estradiol on oocyte maturity and endometrial thickness among unexplained infertile females during ICSI

• Published in: PloS one Vol. 15; no. 10; p. e0239142
• Main Authors: Rehman, Rehana, Zafar, Amara, Ali, Arzina Aziz, Baig, Mukhtiar, Alam, Faiza
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 28.10.2020; Public Library of Science (PLoS)
• Subjects: 17β-Estradiol; Abortion; Biological research; Biology and Life Sciences; Correlation; Embryo transfer; Embryos; Endometrium; Enzyme-linked immunosorbent assay; Females; Fertilization; Follicular fluid; Gametocytes; Genetic aspects; Health aspects; Health care facilities; Hormones; Human artificial insemination; Infertility; Kiss1 protein; Maturity; Medical examination; Medicine and Health Sciences; Metabolism; Oocytes; Ovaries; Ovulation; Pregnancy; Puberty; Reproduction; Retrieval; Sex hormones; Signaling peptides and proteins; Sperm; Stimulation; Thickness; Ultrasonic imaging; Vagina; Pakistan; Karachi Pakistan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0239142

To relate serum and follicular fluid (FF) kisspeptin and estradiol levels in different stages of stimulation during Intracytoplasmic Sperm Injection (ICSI) with oocyte maturity and endometrial thickness among unexplained infertile females. This cross-sectional study was carried out at the Australian Concept Infertility Medical Centre from March 2017 till March 2018. Fifty unexplained infertile females, booked for ICSI, were included in the study. Serum kisspeptin and estradiol were estimated by Enzyme-Linked Immunosorbent Assay in all four stages; 1: follicular stimulation, 2: ovulation induction, 3: oocyte pickup, and 4: embryo transfer. FF was aspirated during oocyte retrieval (stage 3) for the analysis of KP and estradiol. Pregnancy outcomes were categorized as non-pregnant, preclinical abortion, and clinical pregnancy. The age of the study subjects was 32.04 ± 2.29 (Mean±SD) years, with mean BMI of 28.51 ± 4.15 (Mean±SD) kg/m.sup.2 . Mean serum kisspeptin and estradiol levels increased in all subjects as the stimulation proceeded stages 1-3; however, the mean dropped after retrieval of the oocytes (stage 4). Out of 27 female subjects who completed the cycle, 17 remained non-pregnant, 4 had preclinical abortion, and 6 acquired clinical pregnancy. The FF kisspeptin concentration was significantly higher than serum concentrations and positively correlated with serum and FF estradiol concentrations. FF-kisspeptin correlated with serum kisspeptin in Stage 3 (r = 0.930, p<0.001), maturity of oocyte (r = 0.511, p = 0.006) and endometrial thickness (r = 0.522, p = 0.005). Kisspeptin in stage 3 was also found to correlate with endometrial thickness (r = 0.527, p = 0.005) and with estradiol (r = 0.624, p = 0.001) independently. Increase in serum and FF-kisspeptin and estradiol levels from stages 1 to 3, resulted in an optimum endometrial thickness, probability of fertilization of oocytes and chances of clinical pregnancy in Assisted Reproductive Techniques /ICSI cycles of unexplained infertile females.