Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli -mediated diarrhea in pigs

• Published in: Vaccine Vol. 33; no. 39; pp. 5204 - 5211
• Main Authors: Takeyama, Natsumi, Yuki, Yoshikazu, Tokuhara, Daisuke, Oroku, Kazuki, Mejima, Mio, Kurokawa, Shiho, Kuroda, Masaharu, Kodama, Toshiaki, Nagai, Shinya, Ueda, Susumu, Kiyono, Hiroshi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 22.09.2015; Elsevier Limited
• Subjects: Administration, Oral; Allergy and Immunology; Amino acids; Animals; Antibodies, Bacterial - blood; Atmospheric pressure; Binomial distribution; Buoyancy; Colostrum - immunology; Demonstrations & protests; Diarrhea - prevention & control; Diarrhea - veterinary; E coli; Enterotoxigenic Escherichia coli - genetics; Enterotoxigenic Escherichia coli - immunology; Escherichia coli; Escherichia coli Infections - prevention & control; Escherichia coli Infections - veterinary; Escherichia coli Vaccines - administration & dosage; Escherichia coli Vaccines - genetics; Escherichia coli Vaccines - immunology; Female; Hogs; Immunity, Mucosal; Immunization; Immunization, Passive; Immunoglobulin A - blood; Immunoglobulin G - blood; Memorials & monuments; Milk - immunology; Mucosal immunity; Oral vaccine; Oryza - genetics; Pig ETEC; Plant vaccine; Pregnancy; Serum - immunology; Stone; Swine; Swine Diseases - prevention & control; Toxins; Unexplained phenomena; Vaccination; Vaccines; Vaccines, Edible - administration & dosage; Vaccines, Edible - genetics; Vaccines, Edible - immunology; Waterborne diseases; Witchcraft; Japan; heat stable toxin; Mucosal immunity; cholera toxin; monosialotetrahexosylganglioside; LT; GM1; Oral vaccine; 3,3′,5,5′-tetramethylbenzidine; Pig ETEC; CFU; cholera toxin B subunit; Tween 20 PBS; secretory IgA; WT; ST; heat labile toxin B subunit; heat labile toxin; TMB; Plant vaccine; ETEC; CTB; CT; CAYE; colony forming unit; enterotoxigenic Escherichia coli; SIgA; PBST; wild type; LTB; Casamino acids–yeast extract
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2015.07.074

Highlights • MucoRice-CTB is the first veterinary purification-free rice-based oral vaccine. • Oral MucoRice-CTB induces cross-protective immunity to neutralize LT induced by ETEC. • Oral MucoRice-CTB protects pigs from diarrhea caused by ETEC at all life stages.