Binding of the transcription factor MYC2-like to the ABRE of the OsCYP2 promoter enhances salt tolerance in Oryza sativa

Cyclophilins, a type of peptidyl-prolyl cis-trans isomerase, function as important molecular chaperones in a series of biological processes. However, the expression pattern and signal transduction pathway of cyclophilins are still unclear. Here, we showed that the promoter of OsCYP2 could function a...

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Published inPloS one Vol. 17; no. 10; p. e0276075
Main Authors Liu, Hongbo, Cui, Peng, Zhang, Bingxin, Zhu, Jinbo, Liu, Cui, Li, Qingyang
Format Journal Article
LanguageEnglish
Published San Francisco Public Library of Science 14.10.2022
Public Library of Science (PLoS)
Subjects
Abiotic stress
Abscisic acid
Analysis
Biological activity
Biology and life sciences
Cloning
Crop yields
Crops
Ecology and Environmental Sciences
Electrophoretic mobility
Environmental aspects
Enzymes
Genetic transcription
Management
Peptidylprolyl isomerase
Physiology
Plasmids
Potassium
Regulatory sequences
Research and Analysis Methods
Rice
RNA-mediated interference
Salinity tolerance
Salt
Salt tolerance
Seeds
Signal processing
Signal transduction
Statistical analysis
Transcription factors
Yeasts
China
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Summary:Cyclophilins, a type of peptidyl-prolyl cis-trans isomerase, function as important molecular chaperones in a series of biological processes. However, the expression pattern and signal transduction pathway of cyclophilins are still unclear. Here, we showed that the promoter of OsCYP2 could function as a tissue-specific promoter by GUS staining. Moreover, we found that the promoter sequence contained not only core elements but also inducible elements. Then, the ABA-responsive element was used for cDNA library screening, and the transcription factor MYC2-like was identified by a yeast one-hybrid assay and confirmed through an electrophoretic mobility shift assay. Furthermore, the relative expression showed that MYC2-like was induced by abscisic acid. In addition, MYC2-like overexpression enhanced salt tolerance in transformants and partially restored the cyp2-RNAi line. In summary, we explored a novel transcriptional signal mediated by MYC2-like, a potential regulator of salt stress-related physiological processes in rice.
Bibliography:ObjectType-Article-1
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Competing Interests: The authors declare that they have no competing interests.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0276075