Easy and effective analytical method of carbendazim, dimethomorph, and fenoxanil from Protaetia brevitarsis seulensis using LC-MS/MS

• Published in: PloS one Vol. 16; no. 10; p. e0258266
• Main Authors: Baek, Sujin, Noh, Hyun Ho, Kim, Chang Jo, Son, Kyungae, Lee, Hee-Dong, Kim, Leesun
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 14.10.2021; Public Library of Science (PLoS)
• Subjects: Acetonitrile; Biology and Life Sciences; Carbendazim; Chromatography; Composts; Dietary supplements; Evaluation; Farms; Fermented food; Food; Formic acid; Fruits; Fungicides; Insects; Insects as food; Lipids; Liquid chromatography; Management; Mass spectrometry; Mass spectroscopy; Medicine and Health Sciences; Optimization; Pesticides; Physical Sciences; Protaetia brevitarsis; Proteins; Recovery; Research and Analysis Methods; Rural development; Sawdust; Scientific imaging; Target detection; South Korea; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0258266

Traditionally in Korea, Protaetia brevitarsis seulensis (white-spotted flower chafer) has been used as a medicine, and recently has attracted increased attention due to its antithrombotic efficacy. Some of spent mushroom compost or fermented oak sawdust, a feedstock for P. brevitarsis, were contaminated with three fungicides, carbendazim, dimethomorph, and fenoxanil, which could be transferred to the insect. This study was aimed to optimize a simple extraction method combined with liquid chromatography tandem mass spectrometry and apply it to the real samples. After the pulverized samples (5 g) were extracted with acetonitrile (10 mL) and formic acid (100 [mu]L), fat and lipids in the samples were slowly precipitated at -20°C for 24 hours. After eight different clean-up methods were investigated, the mixture of 150 mg MgSO.sub.4 /25 mg PSA/25 mg C18 was selected due to optimal recovery of the target compounds. Recovery (77.9%-80.8% for carbendazim, 111.2%-116.7% for dimethomorph, and 111.9%-112.5% for fenoxanil) was achieved with reasonable relative standard deviation (<5.5%) The analytical method developed in this study was used to analyze three compounds in the 24 insect samples donated by the insect farm owners but no target compounds were detected. These results can provide important data for establishing the pesticide safety standards for P. brevitarsis before the medical applications.