Effect of a Novel Ascorbic Derivative, Disodium Isostearyl 2-O-L-Ascorbyl Phosphate, on Normal Human Dermal Fibroblasts against Reactive Oxygen Species

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 4; pp. 1015 - 1022
• Main Authors: SHIBAYAMA, Hiroharu, HISAMA, Masayoshi, MATSUDA, Sanae, KAWASE, Atsushi, OHTSUKI, Mamitaro, HANADA, Katsumi, IWAKI, Masahiro
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.04.2008; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: amphiphilic vitamin C derivative; Animals; Antioxidants - pharmacology; Ascorbic Acid - analogs & derivatives; Ascorbic Acid - analysis; Ascorbic Acid - pharmacology; Biological and medical sciences; Cattle; Cell Survival - drug effects; Collagen - metabolism; Dermis - cytology; disodium isostearyl 2-O-L-ascorbyl phosphate; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - radiation effects; Fundamental and applied biological sciences. Psychology; human skin fibroblast; Humans; Matrix Metalloproteinase 1 - metabolism; oxidative stress; Oxidative Stress - drug effects; Reactive Oxygen Species - metabolism; Ultraviolet Rays; VCP-IS-2Na; Human; Phosphates; Oxidative stress; Oxygen; disodium isostearyl 2-O-L-ascorbyl phosphate; Vitamin; VCP-IS- 2Na; Skin; amphiphilic vitamin C derivative; Fibroblast; human skin fibroblast
• ISSN: 0916-8451; 1347-6947; 1347-6947
• DOI: 10.1271/bbb.70766

The novel amphiphilic vitamin C derivative disodium isostearyl 2-O-L-ascorbyl phosphate (VCP-IS-2Na), which has a C 18 alkyl chain attached to the stable ascorbate derivative sodium L-ascorbic acid 2-phosphate (VCP-Na), was evaluated for reduction of cell damage induced by oxidative stress, ultraviolet A (UVA), ultraviolet B (UVB), and H 2 O 2 ; stimulation of collagen synthesis against UVA irradiation; and inhibition of matrix metalloproteinase-1 (MMP-1) activity induced by UVA in human normal dermal fibroblasts. VCP-IS-2Na pretreatment resulted in significant protection against cell damage induced by UVB, UVA, and H 2 O 2 . The amount of type I collagen following UVA irradiation was increased by treatment with VCP-IS-2Na in a concentration-dependent manner. These effects of VCP-IS-2Na were superior to those of L-ascorbic acid (vitamin C, VC) and VCP-Na. On the other hand, VCP-IS-2Na suppressed 65% of the excess MMP-1 irradiated UVA, and VC and VCP-Na slightly suppressed it.