Serotonin receptor activity is necessary for olfactory learning and memory in Drosophila melanogaster

• Published in: Neuroscience Vol. 192; pp. 372 - 381
• Main Authors: Johnson, O, Becnel, J, Nichols, C.D
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 29.09.2011; Elsevier
• Subjects: 5-HT1A; 5-HT2; 5-HT7; Animals; Biological and medical sciences; Brain - physiology; Conditioning, Classical; Drosophila melanogaster; Drosophila melanogaster - physiology; ellipsoid body; Female; Fundamental and applied biological sciences. Psychology; Learning - physiology; learning and memory; Male; Memory - physiology; Neurology; Receptors, Serotonin - physiology; serotonin; Smell - physiology; Vertebrates: nervous system and sense organs; LTM; 4-methylcyclohexanol; ( R)-1-[2,5-dimethoxy-4-iodophenyl]-2-aminopropane; short-term memory; long-term memory; STM; CS; mushroom bodies; serotonin; benzaldehyde; ellipsoid body; MCH; learning and memory; G-protein coupled receptor; MB; 5-HT 7; GPCR; 5-HT 2; ( R)-DOI; 5-HT 1A; EB; Canton-S; BA; (R)-DOI; 5-HT2; 5-HT7; 5-HT1A; Serotonin; Insecta; Memory; Serotonine receptor; Drosophilidae; Learning; Acquisition process; Arthropoda; Neurotransmitter; Invertebrata; 5-HT; Drosophila melanogaster; Diptera
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2011.06.058

Abstract Learning and memory in the fruit fly, Drosophila melanogaster , is a complex behavior with many parallels to mammalian learning and memory. Although many neurotransmitters including acetylcholine, dopamine, glutamate, and GABA have previously been demonstrated to be involved in aversive olfactory learning and memory, the role of serotonin has not been well defined. Here, we present the first evidence of the involvement of individual serotonin receptors in olfactory learning and memory in the fly. We initially followed a pharmacological approach, utilizing serotonin receptor agonists and antagonists to demonstrate that all serotonin receptor families present in the fly are necessary for short-term learning and memory. Isobolographic analysis utilizing combinations of drugs revealed functional interactions are occurring between 5-HT1A -like and 5-HT2 , and 5-HT2 and 5-HT7 receptor circuits in mediating short-term learning and memory. Examination of long-term memory suggests that 5-HT1A -like receptors are necessary for consolidation and important for recall, 5-HT2 receptors are important for consolidation and recall, and 5-HT7 receptors are involved in all three phases. Importantly, we have validated our pharmacological results with genetic experiments and showed that hypomorph strains for 5-HT2 Dro and 5-HT1B Dro receptors, as well as knockdown of 5-HT7 Dro mRNA, significantly impair performance in short-term memory. Our data highlight the importance of the serotonin system and individual serotonin receptors to influence olfactory learning and memory in the fly, and position the fly as a model system to study the role of serotonin in cognitive processes relevant to mammalian CNS function.