Inter‐laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191)

• Published in: Environmental toxicology and chemistry Vol. 33; no. 3; pp. 662 - 670
• Main Authors: Feiler, Ute, Ratte, Monika, Arts, Gertie, Bazin, Christine, Brauer, Frank, Casado, Carmen, Dören, Laszlo, Eklund, Britta, Gilberg, Daniel, Grote, Matthias, Gonsior, Guido, Hafner, Christoph, Kopf, Willi, Lemnitzer, Bernd, Liedtke, Anja, Matthias, Uwe, Okos, Ewa, Pandard, Pascal, Scheerbaum, Dirk, Schmitt‐Jansen, Mechthild, Stewart, Kathleen, Teodorovic, Ivana, Wenzel, Andrea, Pluta, Hans‐Jürgen
• Format: Journal Article
• Language: English
• Published: Pensacola, FL Blackwell Publishing Ltd 01.03.2014; SETAC; Wiley
• Subjects: Animal, plant and microbial ecology; Applied ecology; Aquatic Organisms; Aquatic plants; assay; Bioassays; Biological and medical sciences; Chlorophenols - toxicity; Contact; Control equipment; Criteria; ecotoxicological assessment; Ecotoxicology; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on plants and fungi; Flowers & plants; fresh-water sediments; Fundamental and applied biological sciences. Psychology; Geologic Sediments - analysis; Growth rate; growth retardation; Hydrology; Inhibition; Inter-laboratory trial; international organizations; International standards; Life Sciences; Macrophytes; Magnoliopsida - drug effects; Magnoliopsida - growth & development; Myriophyllum aquaticum; quality; Reference Standards; Reproducibility of Results; river; Sediment contact test; Sediment toxicity; Sediments; Toxicity; toxicity testing; Toxicity Tests - methods; Toxicity Tests - standards; Toxicology; variability; Water Pollutants, Chemical - standards; Water Pollutants, Chemical - toxicity; Laboratory study; Haloragidaceae; Toxicity; Myriophyllum aquaticum; Round robin test; Sediments; Sediment contact test; Pollution; Macrophytes; Phytotoxicity; Dicotyledones; Angiospermae; Spermatophyta; ISO standard; Aquatic plant; Inter-laboratory trial; Sediment toxicity; SEDIMENT TOXICITY; MACROPHYTES; MYRIOPHYLLUM AQUATICUM; INTER-LABORATORY TRIAL; SEDIMENT CONTACT TEST
• ISSN: 0730-7268; 1552-8618; 1552-8618
• DOI: 10.1002/etc.2483

A whole‐sediment toxicity test with Myriophyllum aquaticum has been developed by the German Federal Institute of Hydrology and standardized within the International Organization for Standardization (ISO; ISO 16191). An international ring‐test was performed to evaluate the precision of the test method. Four sediments (artificial, natural) were tested. Test duration was 10 d, and test endpoint was inhibition of growth rate (r) based on fresh weight data. Eighteen of 21 laboratories met the validity criterion of r ≥ 0.09 d⁻¹ in the control. Results from 4 tests that did not conform to test‐performance criteria were excluded from statistical evaluation. The inter‐laboratory variability of growth rates (20.6%–25.0%) and inhibition (26.6%–39.9%) was comparable with the variability of other standardized bioassays. The mean test‐internal variability of the controls was low (7% [control], 9.7% [solvent control]), yielding a high discriminatory power of the given test design (median minimum detectable differences [MDD] 13% to 15%). To ensure these MDDs, an additional validity criterion of CV ≤ 15% of the growth rate in the controls was recommended. As a positive control, 90 mg 3,5‐dichlorophenol/kg sediment dry mass was tested. The range of the expected growth inhibition was proposed to be 35 ± 15%. The ring test results demonstrated the reliability of the ISO 16191 toxicity test and its suitability as a tool to assess the toxicity of sediment and dredged material. Environ Toxicol Chem 2014;33:662–670. © 2013 SETAC