Inhibition of Borna disease virus replication by an endogenous bornavirus-like element in the ground squirrel genome

Significance Sequences derived from ancient viruses have been shown to make up a substantial part of animal genomes. Bornaviruses, a genus of nonsegmented, negative-sense RNA virus, also have left their DNA copies in the genomes of a number of vertebrate lineages. Recent studies have demonstrated th...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 111; no. 36; pp. 13175 - 13180
Main Authors Fujino, Kan, Horie, Masayuki, Honda, Tomoyuki, Merriman, Dana K., Tomonaga, Keizo
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 09.09.2014
National Acad Sciences
Subjects
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biological Sciences
Borna Disease - transmission
Borna Disease - virology
Borna disease virus
Borna disease virus - physiology
Cell lines
Cercopithecus aethiops
Conserved Sequence - genetics
DNA
DNA-Directed DNA Polymerase - metabolism
evolution
genome
Genome - genetics
Genomes
HEK293 Cells
hosts
Humans
Infections
Molecular Sequence Data
Mononegavirales
Protein Structure, Tertiary
Proteins
Replicon - genetics
Retroviridae
Retrovirus
Ribonucleoproteins - metabolism
RNA
Sciuridae
Sciuridae - genetics
Sciuridae - virology
Small mammals
Spermophilus
Transfection
Vero Cells
vertebrates
Viral Proteins - chemistry
Viral Proteins - metabolism
virus replication
Virus Replication - genetics
Viruses
antiviral immunity
endogenous nonretroviral viruses
Online AccessGet full text

Cover

More Information
Summary:Significance Sequences derived from ancient viruses have been shown to make up a substantial part of animal genomes. Bornaviruses, a genus of nonsegmented, negative-sense RNA virus, also have left their DNA copies in the genomes of a number of vertebrate lineages. Recent studies have demonstrated that some endogenous bornavirus-like elements (EBLs) may have acquired functions in their hosts as a result of exaptation. In this study, we show that protein encoded by an EBL in the genome of the thirteen-lined ground squirrel efficiently blocks infection and replication of extant bornavirus. To our knowledge, this is the first report showing that endogenous nonretroviral RNA virus elements may function in antiviral defense, providing a potential role for RNA virus endogenization in host evolution.
Bibliography:http://dx.doi.org/10.1073/pnas.1407046111
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 14
ObjectType-Article-1
ObjectType-Feature-2
content type line 23
Author contributions: K.F. and K.T. designed research; K.F., M.H., T.H., D.K.M., and K.T. performed research; D.K.M. contributed new reagents/analytic tools; M.H., T.H., and K.T. analyzed data; and K.F. and K.T. wrote the paper.
Edited* by John M. Coffin, Tufts University School of Medicine, Boston, MA, and approved July 24, 2014 (received for review April 17, 2014)
1Present address; Department of Microbiology II, School of Veterinary Medicine, Azabu University, Fuchinobe, Chuo-ku, Sagamihara-shi, Kanagawa 252-5201, Japan.
2Present address; Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima 890-0065, Japan.
ISSN:0027-8424
1091-6490
1091-6490
DOI:10.1073/pnas.1407046111