Single nucleotide polymorphisms in an STS region linked to the Ncc-tmp1A locus are informative for characterizing the differentiation of chromosome 1A in wheat

Homoeoalleles ofNcc confer nucleus-cytoplasm (NC) compatibility on NC hybrids of wheat with the D plasmon ofAegilops squarrosa. To dissect the chromosomal region containing Ncc, a RAPD marker linked to the Ncc-tmplA locus, which is located on chromosome 1A of T. timopheevi, was sequenced and convert...

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Published inGenes & Genetic Systems Vol. 76; no. 5; pp. 295 - 304
Main Authors Asakura, N. (Kanagawa Univ., Yokohama (Japan)), Mori, N, Ishido, T, Ohtsuka, I, Nakamura, C
Format Journal Article
LanguageEnglish
Published Japan The Genetics Society of Japan 01.10.2001
Japan Science and Technology Agency
Subjects
AEGILOPS SQUARROSA
Alleles
Base Sequence
Biomarkers
Cell Nucleus - genetics
Chromosome 1A
Cytoplasm - genetics
DNA
Genes, Plant
GENETIC MARKERS
LOCI
Molecular Sequence Data
Ncc gene
Ploidies
Polymorphism, Single Nucleotide - genetics
RAPD
Restriction Mapping
TRITICUM
Triticum - genetics
Triticum boeoticum
Triticum timopheevii
Triticum turgidum
Triticum urartu
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Summary:Homoeoalleles ofNcc confer nucleus-cytoplasm (NC) compatibility on NC hybrids of wheat with the D plasmon ofAegilops squarrosa. To dissect the chromosomal region containing Ncc, a RAPD marker linked to the Ncc-tmplA locus, which is located on chromosome 1A of T. timopheevi, was sequenced and converted to a PCR-based sequence-tagged-site (STS) marker. Five single nucleotide polymorphisms (SNPs) between T. timopheevi and T. turgidum. were detected in a 509-bp genomic DNA fragment. Based on the SNPs, the STS alleles in 164 accessions from emmer wheat, timopheevi wheat and two einkorn wheats, T. urartu and T. boeoticum were surveyed by PCR-RFLP analysis. The sequence comparisons and PCR-RFLP analyses revealed nine alleles based on six SNPs. These SNPs were highly conserved within each group of wheat, and all groups could be distinguished by particular combinations of the SNPs. All accessions of T. urartu had one unique STS allele as compared with the others. Our results indicate that the SNPs in the STS marker linked to the Ncc-tmplA locus would be informative for studies of the differentiation of chromosome 1A in wheat.
Bibliography:2002003785
F30
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ISSN:1341-7568
1880-5779
DOI:10.1266/ggs.76.295