The Rise of CRISPR/Cas for Genome Editing in Stem Cells

Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. To cr...

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Bibliographic Details
Published inStem cells international Vol. 2016; no. 2016; pp. 1 - 17
Main Authors Peng, Ying, Guo, Yi, Hernandez Matias, Liz, Shui, Bing
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2016
John Wiley & Sons, Inc
Hindawi Limited
Subjects
Adaptation
Adipocytes
Analysis
Biology
Deoxyribonucleic acid
Design
DNA
Editing
Engineering
Genetic aspects
Genetic engineering
Genomes
Genomics
Laboratories
Medical research
Metabolic disorders
Obesity
Precision medicine
Proteins
Review
Stem cell research
Stem cells
Target recognition
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Summary:Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. To create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology. This review addresses this need directly by providing both the up-to-date biochemical rationale of CRISPR-mediated genome engineering and detailed practical guidelines for the design and execution of CRISPR experiments in cell models. Ultimately, this review will serve as a timely and comprehensive guide for this fast developing technology.
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Academic Editor: Jane Synnergren
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.1155/2016/8140168