Characterization of a CENP-C homolog in Arabidopsis thaliana

• Published in: Genes & Genetic Systems Vol. 79; no. 3; pp. 139 - 144
• Main Authors: Ogura, Y. (Okayama Univ., Kurashiki (Japan). Research Inst. for Bioresources), Shibata, F, Sato, H, Murata, M
• Format: Journal Article
• Language: English
• Published: Japan The Genetics Society of Japan 01.06.2004; Japan Science and Technology Agency
• Subjects: 180-bp repeat; Amino Acid Sequence; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; ARABIDOPSIS THALIANA; Cell Cycle - genetics; CENP-C; centromere; Centromere - metabolism; Chromosomal Proteins, Non-Histone - genetics; Chromosomal Proteins, Non-Histone - metabolism; CHROMOSOMES; Cloning, Molecular; Conserved Sequence; DNA; IMMUNOFLUORESCENCE; In Situ Hybridization, Fluorescence; MOLECULAR CLONING; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; PCR; RNA; Sequence Homology, Amino Acid
• ISSN: 1341-7568; 1880-5779
• DOI: 10.1266/ggs.79.139

Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana, a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.