The augmenting activity of 6-(Methylsulfinyl)hexyl isothiocyanate on cellular glutathione levels is less sensitive to thiol compounds than its cytotoxic activity

• Published in: Bioscience, biotechnology, and biochemistry Vol. 73; no. 6; pp. 1419 - 1421
• Main Authors: Kidachi, Y.(Aomori Univ. (Japan)), Noshita, T, Yamaguchi, H, Umetsu, H, Fuke, Y, Ryoyama, K
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.06.2009; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC); Animals; ARN MENSAJERO; ARN MESSAGER; Biological and medical sciences; CELL CULTURE; Cell Line; Cell Survival - drug effects; CELLS; CELLULE; CELULAS; COMPOSE ORGANIQUE; COMPUESTOS ORGANICOS; CULTIVO DE CELULAS; CULTURE DE CELLULE; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; gamma-glutamylcysteine ligase gene; GENE EXPRESSION; GLUTATHION; GLUTATHIONE; Glutathione - metabolism; GLUTATION; Isothiocyanates - pharmacology; MESSENGER RNA; Mice; ORGANIC COMPOUNDS; Sulfhydryl Compounds - pharmacology; THIOCYANATE; THIOCYANATES; THIOL; thiol compounds; THIOLS; TIOCIANATOS; TIOLES; TOXICIDAD; TOXICITE; TOXICITY; VIABILIDAD; VIABILITE; VIABILITY; Wasabia japonica; gamma-glutamylcysteine ligase gene; Thiol; Gene; 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC); Enzyme; Ligases; Cytotoxicity; Wasabia japonica; thiol compounds; Isothiocyanates; Glutathione
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80791

We analyzed the effects of thiol compounds on the biological activities of 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC). Thiol compounds abolished the cytotoxic activity of 6-MITC, but did not abolish its activity augmenting cellular total glutathione levels and gamma-glutamylcysteine ligase gene expression. Thiol compounds might play an important role in the augmentation of several significant biological activities by overcoming the inherent limitations of 6-MITC.