Interaction between chitosan and oil under stomach and duodenal digestive chemical conditions

• Published in: Bioscience, biotechnology, and biochemistry Vol. 69; no. 11; pp. 2057 - 2062
• Main Authors: Rodriguez, M.S.(Universidad Nacional del Sur, Bahia Blanca (Argentina)), Albertengo, L.E
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.11.2005; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ACEITES; AGITACION; AGITATING; AGITATION; Biological and medical sciences; Chemical Precipitation; CHITOSAN; Chitosan - metabolism; CHITOSANE; Dietary Fats, Unsaturated - metabolism; dietary lipid; DIGESTION; Duodenum - metabolism; EMULSION; EMULSIONS; ESTOMAC; ESTOMAGO; Flocculation; Fundamental and applied biological sciences. Psychology; HUILE; Humans; Hydrogen-Ion Concentration; interaction digestive tract; INTESTIN; Intestinal Absorption; INTESTINES; INTESTINOS; MELANGE; MEZCLADO; MICROSCOPIA; MICROSCOPIE; MICROSCOPY; MIXING; Models, Biological; OILS; QUITOSANO; STOMACH; Stomach - metabolism; water in oil in water emulsion (w/o/w); Water; Stomach; Water oil emulsion; dietary lipid; Diet; Oil water emulsion; interaction digestive tract; Interaction; Lipids; Chitosan; Digestive tract; water in oil in water emulsion (w/o/w)
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.69.2057

Chitosan, the N acetylated derivative of chitin, has an effect on the absorption of dietary lipids, but there is not enough scientific knowledge about the mechanism. To study the interaction between chitosan and oil, the action of this biopolymer has been evaluated through an experimental model of the stomach and duodenum tract, although the enzimatic activity had not been evaluated. We microscopically confirmed that chitosan in a hychloridic acid medium (pH 1.0-2.0) emulsified lipids and the emulsion was a water in oil in water type (w/o/w). When the pH value and speed of agitation were increased to mirror the duodenum medium conditions under which lipids are absorbed, the emulsion capacity was better with an increased number of droplets and the emulsion continued as the w/o/w type. At pH 6.2, chitosan precipitated and lipids were entrapped in the formed flocculus. The binding oil was quantitatively determined, and we also demonstrate that a larger oil quantity induced less retention, while the chitosan characteristics had no influence. These observations allow us to postulate that the interaction between chitosan and oil inhibited duodenal absorption and enhanced lipid excretion.