Measurement of naturally acquired humoral immune responses against the C-terminal region of the Plasmodium vivax MSP1 protein using protein arrays

• Published in: Parasitology research (1987) Vol. 109; no. 5; pp. 1259 - 1266
• Main Authors: Chen, Jun-Hu, Wang, Yue, Ha, Kwon-Soo, Lu, Feng, Suh, In-Bum, Lim, Chae Seung, Park, Jeong Hyun, Takeo, Satoru, Tsuboi, Takafumi, Han, Eun-Taek
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.11.2011; Springer; Springer Nature B.V
• Subjects: Analysis; Antibodies; Antibodies, Protozoan; Antibodies, Protozoan - blood; Antibody response; Antigens, Protozoan; Antigens, Protozoan - immunology; Arrays; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; blood; blood proteins; Control; Criminal investigation; Dosage and administration; Enzyme-linked immunosorbent assay; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Health aspects; Humans; humoral immunity; Identification and classification; Immune response; Immune response (humoral); Immunologic Techniques; Immunologic Techniques - methods; Immunology; Invertebrates; Malaria; Malaria - immunology; Malaria - parasitology; Malaria vaccine; Medical Microbiology; Merozoite Surface Protein 1; Merozoite Surface Protein 1 - immunology; methods; Microbiology; Original Paper; parasitology; pathogens; Plasmodium vivax; Plasmodium vivax - immunology; Prevention; Protein Array Analysis; Protein Array Analysis - methods; Protein arrays; Proteins; recombinant proteins; Risk factors; surface proteins; Vaccine development; Japan; Vivax Infection; Seron Sample; Malaria; Vivax Malaria; Protein Array; Parasite; Protozoa; Plasmodium vivax; Apicomplexa; Humoral immunity; Protein
• ISSN: 0932-0113; 1432-1955; 1432-1955
• DOI: 10.1007/s00436-011-2370-z

Protein arrays are powerful tools for antibody profiling and vaccine development against infectious agents. In the previous report, we successfully applied an antibody-based protein array for immunoprofiling of Plasmodium vivax infection. Herein, we developed a Ni-NTA surface based protein array to detect immune responses against the recombinant C-terminal region (19 and 42 kDa) of the P. vivax merozoite surface protein 1 (PvMSP1-19 and -42) from sera of vivax malaria patients. The PvMSP1-19 arrays detected P. vivax in 112 of 130 (86.2%; 95% CI, 83.2–89.2%) microscopically positive samples and 2 false positives were obtained among 100 sera samples from healthy subjects (2.0%; 95% CI, 0.6–3.4%). These results were in concordance with results of enzyme-linked immunosorbent assays (ELISA). Kappa values represented excellent agreement for the recombinant PvMSP1-19 protein against sera samples as measured by protein arrays and ELISA (Kappa = 0.904, 95% CI: 0.849–0.960). The PvMSP1-42 protein arrays detected antibody response in 100 of 130 microscopically positive samples (76.9%; 95% CI, 72.4–86.8%) and 8 false positives were obtained in 100 healthy subjects (8.0%; 95% CI, 2.7–13.3%). There is no significant difference between the fluorescent intensity of antibody response to PvMSP1-19 and PvMSP1-42 in the positive sera samples ( P  > 0.05). The novel protein array platform may be used for profiling naturally acquired humoral immune responses to P. vivax infection.