Thermally induced changes of lipoate acetyltransferase inner core isolated from the Bacillus stearothermophilus pyruvate dehydrogenase complex

• Published in: Bioscience, biotechnology, and biochemistry Vol. 65; no. 3; pp. 698 - 701
• Main Authors: Aso, Y. (Kyushu Univ., Fukuoka (Japan)), Nakajima, A, Meno, K, Ishiguro, M
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.03.2001; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Acetyltransferases - isolation & purification; Acetyltransferases - metabolism; Analytical, structural and metabolic biochemistry; BACILLUS STEAROTHERMOPHILUS; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Culture Media; Dihydrolipoyllysine-Residue Acetyltransferase; Enzyme Stability; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Geobacillus stearothermophilus - drug effects; Geobacillus stearothermophilus - enzymology; Geobacillus stearothermophilus - growth & development; Guanidine - pharmacology; HEAT; Heating; Light; lipoate acetyltransferase; Miscellaneous; Mission oriented research; PYRUVATE DEHYDROGENASE; pyruvate dehydrogenase complex; Pyruvate Dehydrogenase Complex - metabolism; TEMPERATURE RESISTANCE; thermostability; Transferases; Bacillales; Enzymatic activity; Enzyme; Transferases; Pyruvate dehydrogenase (lipoamide); Bacillus stearothermophilus; Bacteria; Oxidoreductases; Bacillaceae; Dihydrolipoamine S-acetyltransferase; Thermal stability
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.65.698

Incubation at 70°C converted the Bacillus stearothermophilus lipoate acetyltransferase inner core into an unidentified active molecular form, X, yielding an inactive aggregate. The core and X showed similar thermostabilities, but they were different in the recovery of enzyme activity after incubation with 1.2-2.0 M guanidine hydrochloride and its subsequent removal; the core was hardly recovered, but X was well recovered.