Production of isocyclomaltopentaose from starch using isocyclomaltooligosaccharide glucanotransferase

• Published in: Bioscience, biotechnology, and biochemistry Vol. 70; no. 12; pp. 3013 - 3018
• Main Authors: Watanabe, H.(Hayashibara Biochemical Labs. Inc., Okayama (Japan). Research Center), Takakura Yamamoto, R, Kurose, M, Yoshida, K, Oku, K, Sawatani, I, Nishimoto, T, Kubota, M, Chaen, H, Fukuda, S
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.12.2006; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ALMIDON; AMIDON; Bacillus - enzymology; BACILLUS CIRCULANS; Biological and medical sciences; CHEMICAL COMPOSITION; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; cyclomaltopentaose; Fundamental and applied biological sciences. Psychology; glucanotransferase; GLUCOSA; GLUCOSE; Glucosyltransferases - metabolism; Humans; Hydrogen-Ion Concentration; Isoamylase - metabolism; Kinetics; OLIGOSACARIDOS; OLIGOSACCHARIDE; OLIGOSACCHARIDES; Oligosaccharides - chemical synthesis; PRODUCCION; PRODUCTION; RENDEMENT; RENDIMIENTO; STARCH; Starch - chemistry; Substrate Specificity; TEMPERATURA; TEMPERATURE; TRANSFERASE; TRANSFERASES; TRANSFERIAS; YIELDS; α-1,6-linkage; cyclomaltopentaose; Bacillales; Bacillus circulans; Starch; Production; glucanotransferase; Bacteria; α-1,6-linkage; Bacillaceae
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.60404

Production of a novel cyclomaltopentaose cyclized by an alpha-1,6-linkage, [ICG5; cyclo-(-6)-alpha-D-Glcp-(1-4)alpha-D-Glcp-(1-4)-alpha-D-Glcp-(1-4)-alpha-D-Glcp-(1-4)-alpha-D-Glcp-(1-)], from starch was performed using isocyclomaltooligosaccharide glucanotransferase (IGTase) derived from Bacillus circulans AM7. The optimal conditions for ICG5-production from partially hydrolyzed starch were as follows: substrate concentration, 1.0% (w/v); pH, 5.5; temperature, 45 deg C; reaction time, 24 h, IGTase, 1.0 unit/g-dry solid (DS); isoamylase, 2500 units/g-DS. The yield of ICG5 reached 25.9% under optimal conditions. ICG5-production was achieved from partially hydrolyzed starch using a crude enzyme preparation containing IGTase. Finally, ICG5 was obtained in a yield of 17.9 % (99.3% purity, 2681 g-DS). A digestive test with a human salivary amylase, an artificial gastric juice, a pancreatic amylase, and small intestinal enzymes showed that ICG5 was an indigestible oligosaccharide.