The Diagnosis of Gastric Mucosa-associated Lymphoid Tissue Lymphoma by Flow Cytometry and Fluorescence in situ Hybridization of Biopsy Specimens

• Published in: Internal Medicine Vol. 57; no. 8; pp. 1081 - 1086
• Main Authors: Matsueda, Katsunori, Omote, Sizuma, Sakata, Masahiro, Fujita, Isao, Horii, Jouichiro, Toyokawa, Tatsuya
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Society of Internal Medicine 01.01.2018; Japan Science and Technology Agency
• Subjects: Aged; Biopsy; Case Report; CD45 antigen; Diagnosis, Differential; Differential diagnosis; Endoscopy; Flow Cytometry; Fluorescence; Fluorescence in situ hybridization; Gastric mucosa; Gating; Humans; In Situ Hybridization, Fluorescence; Inflammation; Internal medicine; Lymphoma; Lymphoma, B-Cell, Marginal Zone - diagnosis; Lymphoma, B-Cell, Marginal Zone - diagnostic imaging; Lymphoma, B-Cell, Marginal Zone - pathology; Male; Malt; mucosa-associated lymphoid tissue lymphoma; Mucosal-associated lymphoid tissue; reactive inflammatory lymphoid changes; Trisomy; trisomy 18; fluorescence in situ hybridization; flow cytometry; mucosa-associated lymphoid tissue lymphoma; reactive inflammatory lymphoid changes; trisomy 18
• ISSN: 0918-2918; 1349-7235
• DOI: 10.2169/internalmedicine.9617-17

Mucosa-associated lymphoid tissue (MALT) lymphoma and reactive inflammatory lymphoid changes are frequently difficult to distinguish based on a routine histological differential diagnosis. We were unable to diagnose gastric MALT lymphoma histologically using specimens obtained by endoscopy, although a flow cytometry (FCM) analysis demonstrated clonality of neoplastic cells by separating cells by CD45 gating. Furthermore, a fluorescence in situ hybridization (FISH) analysis showed trisomy 18. We therefore diagnosed gastric MALT lymphoma with trisomy 18. We recommend that FCM and FISH analyses of biopsy specimens be considered for diagnosing gastric MALT lymphoma if this diagnosis is suspected based on endoscopic findings.