Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology

• Published in: BMC biotechnology Vol. 22; no. 1; p. 1
• Main Authors: Khalilvand, Aida Bakhshi, Aminzadeh, Saeed, Sanati, Mohammad Hossein, Mahboudi, Fereidoun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 03.01.2022; BioMed Central; BMC
• Subjects: Batch culture; Biomass; Carbon sources; CCD; Cell culture; Cell density; Cell growth; Chemical bonds; Culture media; Culture Media - chemistry; Density; Design of experiments; Disulfide bonds; Disulfides; Dosage and administration; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Fermentation; Identification and classification; Inclusion bodies; Insulin; Insulin lispro; Insulin Lispro - metabolism; Magnesium chloride; Media; Medium composition; Metal ions; Methods; Nitrogen sources; Optimization; PBD; pH effects; Proinsulin - genetics; Proteins; Response surface methodology; Response surfaces (Statistics); RSM; Salts; SHuffle; Software; Yeast; Yeasts; Iran; PBD; SHuffle; CCD; RSM; Medium composition; E. coli
• ISSN: 1472-6750; 1472-6750
• DOI: 10.1186/s12896-021-00732-4

SHuffle is a suitable Escherichia coli (E. coli) strain for high yield cytoplasmic soluble expression of disulfide-bonded proteins such as Insulin due to its oxidative cytoplasmic condition and the ability to correct the arrangement of disulfide bonds. Lispro is an Insulin analog that is conventionally produced in E. coli as inclusion bodies (IBs) with prolonged production time and low recovery. Here in this study, we aimed to optimize cultivation media composition for high cell density fermentation of SHuffle T7 E. coli expressing soluble Lispro proinsulin fused to SUMO tag (SU-INS construct) to obtain high cell density fermentation. Factors including carbon and nitrogen sources, salts, metal ions, and pH were screened via Plackett-Burman design for their effectiveness on cell dry weight (CDW) as a measure of cell growth. The most significant variables of the screening experiment were Yeast extract and MgCl concentration, as well as pH. Succeedingly, The Central Composite Design was utilized to further evaluate and optimize the level of significant variables. The Optimized media (OM-I) enhanced biomass by 2.3 fold in the shake flask (2.5 g/L CDW) that reached 6.45 g/L (2.6 fold increase) when applied in batch culture fermentation. The efficacy of OM-I media for soluble expression was confirmed in both shake flask and fermentor. The proposed media was suitable for high cell density fermentation of E. coli SHuffle T7 and was applicable for high yield soluble expression of Lispro proinsulin.