Monoclonal antibody-based capture ELISA in the diagnosis of previous dengue infection

• Published in: Virology journal Vol. 16; no. 1; p. 125
• Main Authors: Sirivichayakul, Chukiat, Limkittikul, Kriengsak, Chanthavanich, Pornthep, Yoksan, Sutee, Ratchatatat, Anuttarasakdi, Lim, Jacqueline Kyungah, Arunsodsai, Watcharee, Sabchareon, Arunee
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 29.10.2019; BioMed Central; BMC
• Subjects: Antibodies; Antibodies, Monoclonal - immunology; Antibodies, Viral - blood; blood serum; Child; Child, Preschool; Children; Cohort Studies; Dengue; Dengue - blood; Dengue - diagnosis; Dengue - epidemiology; Dengue fever; Dengue vaccines; Dengue Virus - immunology; Dengue Virus - isolation & purification; Diagnosis; Distribution; Encephalitis; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - standards; Enzymes; Health aspects; Humans; Immunoglobulin G; Immunoglobulin M - blood; Immunoglobulins; Infection; Japanese encephalitis; Microbiological Techniques - methods; Microbiological Techniques - standards; Monoclonal antibodies; Monoclonal antibody; mosquito-borne diseases; Neutralization Tests; Plaque reduction neutralization test; screening; Sensitivity and Specificity; Seroepidemiologic Studies; seroprevalence; Thailand; Thailand - epidemiology; Vaccination; Vaccines; Thailand; Plaque reduction neutralization test; Monoclonal antibody; Dengue; Enzyme-linked immunosorbent assay
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/s12985-019-1222-9

Dengue is an important mosquito-borne disease. There is currently only one licensed vaccine for dengue prevention. The vaccine provides higher efficacy in pre-vaccination dengue-seropositive persons but a higher risk of subsequent more severe dengue in dengue-seronegative persons. It is recommended that the dengue vaccine may be given in dengue-seropositive individuals or as mass vaccination without individual pre-vaccination screening in areas where the dengue seroprevalence is > 80% in children aged 9 years. We evaluated a dengue specific immunoglobulin G monoclonal antibody-based capture enzyme-linked immunosorbent assay (MAb-ELISA) in the diagnosis of previous dengue infection using serum samples from the cohort study in Ratchaburi Province, Thailand. The MAb-ELISA was compared to 70% plaque reduction neutralization test (PRNT70) in 453 serum samples from children aged 3-11 years in Ratchaburi Province, Thailand. The sensitivity and specificity of MAb-ELISA at the positive to negative (P/N) ratio cut-off level of > 3 were both 0.91 in the diagnosis of previous dengue infection, compared to PRNT70. The false positivity was mainly in Japanese encephalitis (JE) seropositive subjects. This research provides evidence that MAb-ELISA is useful for dengue seroprevalence study and dengue pre-vaccination screening. JE seropositivity was the major cause of false positive result in the study population.