Dose-dependent effect of glucose on GLP-1 secretion involves sweet taste receptor in isolated perfused rat ileum [Rapid Communication]

• Published in: ENDOCRINE JOURNAL Vol. 63; no. 12; pp. 1141 - 1147
• Main Authors: Xu, Zhiwei, Wang, Wendong, Nian, Xiaofeng, Song, Guiqin, Zhang, Xiaoyun, Xiao, Hongyuan, Zhu, Xiaobo
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japan Endocrine Society 2016
• Subjects: Animals; Cells, Cultured; Dipeptidyl Peptidase 4 - metabolism; Dose-Response Relationship, Drug; Glucagon-like peptide 1; Glucagon-Like Peptide 1 - secretion; Glucose - pharmacology; Glucose concentration; Ileum; Ileum - drug effects; Ileum - metabolism; Male; Organ Culture Techniques; Plant Proteins - pharmacology; Rat; Rats; Rats, Sprague-Dawley; Receptors, G-Protein-Coupled - metabolism; Receptors, G-Protein-Coupled - physiology; Signal Transduction - drug effects; Sweet taste receptor
• ISSN: 0918-8959; 1348-4540
• DOI: 10.1507/endocrj.EJ16-0390

Luminal glucose is an important stimulus for glucagon-like peptide 1 (GLP-1) secretion from intestinal endocrine cells. However, the effects of luminal glucose concentration on GLP-1 secretion remain unknown. In this study, we investigated the effect of luminal glucose concentrations (3.5, 5, 10, 15, and 20 mmol/L) on GLP-1 secretion from isolated perfused rat ileum. Results showed that the perfusate glucose concentration dose-dependently stimulated GLP-1 secretion from isolated perfused rat ileum, which was eliminated by the sweet taste receptor inhibitor gurmarin (30 μg/mL), but not inhibited by phloridzin (1 mmol/L), a Na+-coupled glucose transporters inhibitor. We conclude that luminal glucose induced GLP-1 secretion from perfused rat ileum in a concentration-dependent manner. This secretion was mediated by sweet taste receptor transducing signal for GLP-1 release on the gut of rat.