Characterization of two Restriction Endonucleases, SenPT14bI and SenPT16I, in Standard Phage-Type Strains of Salmonella Enteritidis

• Published in: Biological & pharmaceutical bulletin Vol. 20; no. 11; pp. 1212 - 1214
• Main Authors: MIYAHARA, Michiko, NAKAMURA, Akiko, MISE, Katsutoshi
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.11.1997; Maruzen; Japan Science and Technology Agency
• Subjects: Biological and medical sciences; Biotechnology; Chromatography, Ion Exchange; Deoxyribonucleases, Type II Site-Specific - isolation & purification; Deoxyribonucleases, Type II Site-Specific - metabolism; DNA, Viral - metabolism; Enzyme engineering; Fundamental and applied biological sciences. Psychology; Hydrolysis; Improved methods for extraction and purification of enzymes; Methods. Procedures. Technologies; restriction endonuclease; SacII isoschizomer; Salmonella Enteritidis; Salmonella enteritidis - virology; Salmonella Phages - enzymology; XmaIII isoschizomer; Characterization; Salmonella enteritidis; Specificity; Purification; Stability; Enzyme; Gel electrophoresis; Restriction endonuclease; Bacteria; Aminoacid sequence; Enterobacteriaceae
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.20.1212

Two restriction endonucleases (ENases) were found by screening 38 standard phage strains of Salmonella (S.) Enteritidis. An isoschizomer of SacII ENase that recognizes the sequence 5'-CCGC/GG-3' was identified in S. Enteritidis PT14b, and an isoschizomer of XmaIII ENase (5'-C/GGCCG-3') was found in S. Enteritidis PT16.It is of special interest that the recognition specificities of all known ENases in Salmonella, including those of the S. Enteritidis ENases, are very similar to each other.