Functional and spatial segregation of secretory vesicle pools according to vesicle age

Synaptic terminals and neuroendocrine cells are packed with secretory vesicles, only a few of which are docked at the plasma membrane and readily releasable. The remainder are thought to constitute a large cytoplasmic reserve pool awaiting recruitment into the readily releasable pool (RRP) for exocy...

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Published inNature (London) Vol. 422; no. 6928; pp. 176 - 180
Main Authors Shipston, Michael J, Chow, Robert H, Duncan, Rory R, Greaves, Jennifer, Wiegand, Ulrich K, Matskevich, Ioulia, Bodammer, Georg, Apps, David K
Format Journal Article
LanguageEnglish
Published London Nature Publishing 13.03.2003
Nature Publishing Group
Subjects
Adrenal Glands - cytology
Animals
Biological and medical sciences
Biological Transport
Cattle
Cell Membrane
Cell physiology
Cells
Cellular biology
Cellular Senescence
Chromaffin Cells - cytology
Exocytosis
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Movement
Rats
Secretion. Exocytosis
Secretory Vesicles - physiology
Time Factors
Vesicle
Bovine
Adrenal glands
Secretion
Segregation
Ageing
Secretory cell
Chromaffin cell
Exocytosis
Vertebrata
Mammalia
Cell line
Plasma membrane
Artiodactyla
Secretion granule
Ungulata
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Summary:Synaptic terminals and neuroendocrine cells are packed with secretory vesicles, only a few of which are docked at the plasma membrane and readily releasable. The remainder are thought to constitute a large cytoplasmic reserve pool awaiting recruitment into the readily releasable pool (RRP) for exocytosis. How vesicles are prioritized in recruitment is still unknown: the choice could be random, or else the oldest or the newest ones might be favoured. Here we show, using a fluorescent cargo protein that changes colour with time, that vesicles in bovine adrenal chromaffin cells segregate into distinct populations, based on age. Newly assembled vesicles are immobile (morphologically docked) at the plasma membrane shortly after biogenesis, whereas older vesicles are mobile and located deeper in the cell. Different secretagogues selectively release vesicles from the RRP or, surprisingly, selectively from the deeper cytoplasmic pool. Thus, far from being equal, vesicles are segregated functionally and spatially according to age.
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ISSN:0028-0836
1476-4687
DOI:10.1038/nature01389