Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria

• Published in: Applied and Environmental Microbiology Vol. 58; no. 7; pp. 2180 - 2187
• Main Author: De Bruijn, F.J. (Michigan State University, E. Lansing, MI)
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.07.1992
• Subjects: ADN; AGROBACTERIUM RHIZOGENES; AGROBACTERIUM TUMEFACIENS; BACTERIA; Base Sequence; Biological and medical sciences; Biology; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; BRADYRHIZOBIUM; CLASIFICACION; CLASSIFICATION; distribution; DNA; DNA Fingerprinting; DNA, Bacterial - genetics; Escherichia coli - genetics; FLORA DEL SUELO; FLORE DU SOL; Fundamental and applied biological sciences. Psychology; Genetics; Genome, Bacterial; IDENTIFICACION; IDENTIFICATION; Mission oriented research; Molecular Sequence Data; NUCLEOTIDE; NUCLEOTIDOS; Polymerase Chain Reaction; PSEUDOMONAS; Pseudomonas - genetics; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS PUTIDA; repeated sequence; Repetitive Sequences, Nucleic Acid; Rhizobium - genetics; RHIZOBIUM LEGUMINOSARUM; RHIZOBIUM MELILOTI; Sinorhizobium meliloti - classification; Sinorhizobium meliloti - genetics; Soil Microbiology; soil microorganisms; Soils; Species Specificity; Polymerase chain reaction; Soils; Rhizobium meliloti; Consensus sequence; Repeated sequence; Fingerprint method; Bacteria; Isolate; Rhizobiaceae; Genome; Palindromic sequence
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.58.7.2180-2187.1992

The distribution of dispersed repetitive DNA (repetitive extragenic palindromic [REP] and enterobacterial repetitive intergenic consensus [ERIC]) sequences in the genomes of a number of gram-negative soil bacteria was examined by using conserved primers corresponding to REP and ERIC sequences and the polymerase chain reaction (PCR). The patterns of the resulting PCR products were analyzed on agarose gels and found to be highly specific for each strain. The REP and ERIC PCR patterns of a series of Rhizobium meliloti isolates, previously ordered in a phylogenetic tree based on allelic variations at 14 enzyme loci (B. D. Eardly, L. A. Materon, N. H. Smith, D. A. Johnson, M. D. Rumbaugh, and R. K. Selander, Appl. Environ. Microbiol. 56:187-194), were determined. Isolates which had been postulated to be closely related by multilocus enzyme electrophoresis also revealed similar REP and ERIC PCR patterns, suggesting that the REP and ERIC PCR method is useful for the identification and classification of bacterial strains