Identification of mutations conferring streptomycin resistance in multidrug-resistant tuberculosis of China

• Published in: Diagnostic microbiology and infectious disease Vol. 83; no. 2; pp. 150 - 153
• Main Authors: Zhao, Li-li, Liu, Hai-can, Sun, Qing, Xiao, Tong-yang, Zhao, Xiu-qin, Li, Gui-lian, Zeng, Chun-yan, Wan, Kang-lin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2015
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents - pharmacology; China; Female; Genotype; Humans; Infectious Disease; Internal Medicine; Male; Middle Aged; Multidrug-resistant tuberculosis; Mutation; Mycobacterium; Mycobacterium tuberculosis - classification; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - isolation & purification; Streptomycin - pharmacology; Streptomycin resistance; Tuberculosis, Multidrug-Resistant - microbiology; Young Adult; China; Genotype; Multidrug-resistant tuberculosis; Streptomycin resistance; Mutation
• ISSN: 0732-8893; 1879-0070; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2015.06.020

We investigated the spectrum and frequency of mutations in rpsL, rrs, and gidB among 140 multidrug-resistant tuberculosis (MDR-TB) clinical isolates from China. The association between mutations and different genotypes was also analyzed. Our data revealed that 65.7% of MDR-TB were resistant to streptomycin (STR), and 90.2% of STR-resistant isolates were Beijing strains. STR resistance was correlated with Beijing family (P=0.00). Compared with phenotypic data, detection of mutations for the combination of these 3 genes exhibited 94.6% sensitivity, 91.7% specificity, and 93.6% accuracy. The most common mutations in STR-resistant isolates were rpsL128, 262, and rrs514, of which rpsL128 showed association with Beijing lineage (P=0.00). A combination of these 3 mutations can serve as the reliable predictors for STR resistance, showing the sensitivity, specificity, and accuracy of 85.9%, 97.9%, and 90.0%, respectively. Furthermore, gidBA276C, not A615G, was Beijing lineage specific. These findings are useful to develop rapid molecular diagnostic methods for STR resistance in China. •We investigated STR-resistant mutations and genotypes of MDR-TB isolates in China.•DNA sequencing for the detection of STR resistance was evaluated with phenotypic data.•The most common mutations in STR-resistant isolates were rpsL128, 262, and rrs514.•Beijing genotype showed association with STR resistance and rpsL128 mutation.