Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development
Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (K...
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Published in | Animal science journal Vol. 87; no. 3; pp. 457 - 461 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Australia
Blackwell Publishing Ltd
01.03.2016
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Subjects | |
Online Access | Get full text |
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Summary: | Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle. |
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Bibliography: | Grant-in-Aid for Scientific Research (B) to NY - No. 26292141 Frontier Research Award on Agricultural Sciences, Tohoku University, to MK and YH. Part of this work was also supported by Grant-in-Aid for Scientific Research (C) to MT - No. 24580439 istex:5474D326406BA044D2B3A4AD04DC0680875D1F74 ark:/67375/WNG-VFQX2MF8-F ArticleID:ASJ12538 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1344-3941 1740-0929 |
DOI: | 10.1111/asj.12538 |