ATP-dependent potassium channels contribute to motor regulation of esophageal striated muscle in rats

• Published in: Journal of Veterinary Medical Science Vol. 81; no. 9; pp. 1266 - 1272
• Main Authors: HORII, Kazuhiro, SUZUKI, Yuji, SHIINA, Takahiko, SAITO, Shouichiro, ONOUCHI, Sawa, HORII, Yuuki, SHIMAOKA, Hiroki, SHIMIZU, Yasutake
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2019; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Adenosine Triphosphate; Animals; ATP; ATP-dependent potassium channel; Electric Stimulation; Electrical stimuli; Esophagus; Esophagus - drug effects; Esophagus - innervation; Glibenclamide; Glyburide - pharmacology; Male; Minoxidil; Minoxidil - pharmacology; Muscle contraction; Muscle Contraction - drug effects; Muscle Contraction - physiology; Muscle, Striated - drug effects; Muscle, Striated - physiology; Muscles; Physiology; Polymerase chain reaction; Potassium; Potassium Channel Blockers - pharmacology; Potassium channels; Potassium Channels - drug effects; Potassium Channels - physiology; rat; Rats, Sprague-Dawley; Rodents; Skeletal muscle; striated muscle; Vagus nerve; Vagus Nerve - physiology; rat; esophagus; ATP-dependent potassium channel; striated muscle
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.19-0197

The aim of the present study was to clarify roles of ATP-dependent potassium channels (KATP channels) in motility of the striated muscle portion in the esophagus. An isolated segment of the rat esophagus was placed in an organ bath and mechanical responses were recorded using a force transducer. Electrical stimulation of the vagus nerve evoked contractile response of striated muscle in the esophageal segment. Application of glibenclamide, an antagonist of KATP channels, increased amplitude of vagally mediated twitch contractions of the rat esophagus. On the other hand, minoxidil, an agonist of KATP channels, decreased amplitude of twitch contractions. RT-PCR revealed the expression of subunits of KATP channels in esophageal tissue. In addition, immunopositivity for subunits of KATP channels was observed in the striated muscle cells of the esophageal muscle layer. These findings indicate that KATP channels contribute to motor regulation of striated muscle in the rat esophagus.