The effect of oxidant and the non-oxidant alteration of cellular thiol concentration on the formation of protein mixed-disulfides in HEK 293 cells

• Published in: PloS one Vol. 3; no. 12; p. e4015
• Main Authors: Gilge, Jasen Lee, Fisher, Michael, Chai, Yuh-Cherng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 24.12.2008; Public Library of Science (PLoS)
• Subjects: Antioxidants; Antioxidants (Nutrients); Antioxidants - analysis; Antioxidants - pharmacology; Biochemistry/Chemical Biology of the Cell; Biosynthesis; Buthionine sulfoximine; Cell Biology/Cellular Death and Stress Responses; Cell Biology/Chemical Biology of the Cell; Cell Line; Chemistry; Chromatography; Cysteine; Cystine; Disulfides; Disulfides - metabolism; Enzymes; Free radicals; Glutathione; Glutathione - analysis; Glutathione - metabolism; High performance liquid chromatography; Humans; Hydrogen; Hydrogen ion concentration; Hydrogen peroxide; Immunoblotting; Kidney - chemistry; Kidney - cytology; Kidney - embryology; Kidney - metabolism; Kinases; Liquid chromatography; Metabolism; Methionine; Molecular weight; Monoclonal antibodies; Neutrophils; Oxidants; Oxidants - analysis; Oxidants - pharmacology; Oxidation; Oxidative Stress - physiology; Oxidizing agents; Physiological aspects; Physiology; Polymerization; Post-translation; Post-translational modifications; Protein Disulfide-Isomerases - metabolism; Protein Processing, Post-Translational; Protein S; Proteins; Reagents; Rodents; Signal transduction; Signaling; Sulfhydryl Compounds - metabolism; Sulfhydryl Compounds - pharmacology; Thiols; Toxicity; United States > US; Ohio
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0004015

Cellular molecules possess various mechanisms in responding to oxidant stress. In terms of protein responses, protein S-glutathionylation is a unique post-translational modification of protein reactive cysteines forming disulfides with glutathione molecules. This modification has been proposed to play roles in antioxidant, regulatory and signaling in cells under oxidant stress. Recently, the increased level of protein S-glutathionylation has been linked with the development of diseases. In this report, specific S-glutathionylated proteins were demonstrated in human embryonic kidney 293 cells treated with two different oxidative reagents: diamide and hydrogen peroxide. Diamide is a chemical oxidizing agent whereas hydrogen peroxide is a physiological oxidant. Under the experimental conditions, these two oxidants decreased glutathione concentration without toxicity. S-glutathionylated proteins were detected by immunoblotting and glutathione concentrations were determined by high performance liquid chromatography. We further show the effect of alteration of the cellular thiol pool on the amount of protein S-glutathionylation in oxidant-treated cells. Cellular thiol concentrations were altered either by a specific way using buthionine sulfoximine, a specific inhibitor of glutathione biosynthesis or by a non-specific way, incubating cells in cystine-methionine deficient media. Cells only treated with either buthionine sulfoximine or cystine-methionine deficient media did not induce protein S-glutathionylation, even though both conditions decreased 65% of cellular glutathione. Moreover, the amount of protein S-glutathionylation under both conditions in the presence of oxidants was not altered when compared to the amount observed in regular media with oxidants present. Protein S-glutathionylation is a dynamic reaction which depends on the rate of adding and removing glutathione. Phenylarsine oxide, which specifically forms a covalent adduct with vicinal thiols, was used to determine the possible role of vicinal thiols in the amount of glutathionylation. Our data shows phenylarsine oxide did not change glutathione concentrations, but it did enhance the amount of glutathionylation in oxidant-treated cells.