Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway

• Published in: Journal of animal science and biotechnology Vol. 14; no. 1; p. 32
• Main Authors: Joo, Ye Eun, Jeong, Pil-Soo, Lee, Sanghoon, Jeon, Se-Been, Gwon, Min-Ah, Kim, Min Ju, Kang, Hyo-Gu, Song, Bong-Seok, Kim, Sun-Uk, Cho, Seong-Keun, Sim, Bo-Woong
• Format: Journal Article
• Language: English
• Published: England BioMed Central 22.02.2023; Department of Biotechnology,College of Engineering,Daegu University,Gyeongsan,South Korea%Futuristic Animal Resource and Research Center,Korea Research Institute of Bioscience and Biotechnology,Cheongju,South Korea; Futuristic Animal Resource and Research Center,Korea Research Institute of Bioscience and Biotechnology,Cheongju,South Korea; Laboratory of Theriogenology,College of Veterinary Medicine,Chungnam National University,Daejeon,South Korea%Futuristic Animal Resource and Research Center,Korea Research Institute of Bioscience and Biotechnology,Cheongju,South Korea; Department of Animal Science and Biotechnology,College of Agriculture and Life Science,Chungnam National University,Daejeon,South Korea%Futuristic Animal Resource and Research Center,Korea Research Institute of Bioscience and Biotechnology,Cheongju,South Korea; Department of Functional Genomics,University of Science and Technology,Daejeon,South Korea%Department of Animal Science,College of Natural Resources and Life Science,Life and Industry Convergence Research Institute,Pusan National University,Miryang,South Korea; Department of Animal Science,College of Natural Resourcesand Life Science,Pusan National University,Miryang,South Korea%Futuristic Animal Resource and Research Center,Korea Research Institute of Bioscience and Biotechnology,Cheongju,South Korea%Futuristic Animal Resource and Research Center,Korea Research Institute of Bioscience and Biotechnology,Cheongju,South Korea; BMC
• Subjects: Anethole; Antioxidants; Benzene; Cell number; Cell survival; Embryogenesis; Embryonic growth stage; Embryos; Experiments; Fatty acids; Female reproductive system; Glutathione; Hedgehog protein; In vitro fertilization; Infertility; Laboratories; Lipid metabolism; Lipids; Membrane potential; Metabolism; Mitochondria; Mitochondrial function; Oxidative stress; Penicillin; Polyvinyl alcohol; Porcine embryo development; Potassium; Reactive oxygen species; Signal transduction; Signaling; Sonic hedgehog signaling pathway; Survival; Trophectoderm; United States > US; Sonic hedgehog signaling pathway; Anethole; Lipid metabolism; Mitochondrial function; Porcine embryo development
• ISSN: 1674-9782; 2049-1891; 2049-1891
• DOI: 10.1186/s40104-022-00824-x

Anethole (AN) is an organic antioxidant compound with a benzene ring and is expected to have a positive impact on early embryogenesis in mammals. However, no study has examined the effect of AN on porcine embryonic development. Therefore, we investigated the effect of AN on the development of porcine embryos and the underlying mechanism. We cultured porcine in vitro-fertilized embryos in medium with AN (0, 0.3, 0.5, and 1 mg/mL) for 6 d. AN at 0.5 mg/mL significantly increased the blastocyst formation rate, trophectoderm cell number, and cellular survival rate compared to the control. AN-supplemented embryos exhibited significantly lower reactive oxygen species levels and higher glutathione levels than the control. Moreover, AN significantly improved the quantity of mitochondria and mitochondrial membrane potential, and increased the lipid droplet, fatty acid, and ATP levels. Interestingly, the levels of proteins and genes related to the sonic hedgehog (SHH) signaling pathway were significantly increased by AN. These results revealed that AN improved the developmental competence of porcine preimplantation embryos by activating SHH signaling against oxidative stress and could be used for large-scale production of high-quality porcine embryos.