Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

• Published in: Nature biotechnology Vol. 35; no. 8; pp. 725 - 731
• Main Authors: Bowers, Robert M, Kyrpides, Nikos C, Stepanauskas, Ramunas, Harmon-Smith, Miranda, Doud, Devin, Reddy, T B K, Schulz, Frederik, Jarett, Jessica, Rivers, Adam R, Eloe-Fadrosh, Emiley A, Tringe, Susannah G, Ivanova, Natalia N, Copeland, Alex, Clum, Alicia, Becraft, Eric D, Malmstrom, Rex R, Birren, Bruce, Podar, Mircea, Bork, Peer, Weinstock, George M, Garrity, George M, Dodsworth, Jeremy A, Yooseph, Shibu, Sutton, Granger, Glöckner, Frank O, Gilbert, Jack A, Nelson, William C, Hallam, Steven J, Jungbluth, Sean P, Ettema, Thijs J G, Tighe, Scott, Konstantinidis, Konstantinos T, Liu, Wen-Tso, Baker, Brett J, Rattei, Thomas, Eisen, Jonathan A, Hedlund, Brian, McMahon, Katherine D, Fierer, Noah, Knight, Rob, Finn, Rob, Cochrane, Guy, Karsch-Mizrachi, Ilene, Tyson, Gene W, Rinke, Christian, Lapidus, Alla, Meyer, Folker, Yilmaz, Pelin, Parks, Donovan H, Murat Eren, A, Schriml, Lynn, Banfield, Jillian F, Hugenholtz, Philip, Woyke, Tanja
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2017; Nature Publishing Group; Springer Nature
• Subjects: 45; 45/23; 60 APPLIED LIFE SCIENCES; 631/326/171; 706/648/697/129/2043; Agriculture; Archaea; Assembly; Bacteria; BASIC BIOLOGICAL SCIENCES; Bioinformatics; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Check lists; Consortia; Contamination; Gene sequencing; Genetic aspects; genetic markers; genome; Genome, Archaeal - genetics; Genome, Bacterial - genetics; Genomes; Genomic analysis; Genomics; Genomics - methods; Genomics - standards; information; Input output analysis; Laboratories; Life Sciences; Metadata; Metagenomics; Metagenomics - methods; Metagenomics - standards; Microbiological research; Microorganisms; Nucleotide sequence; nucleotide sequences; perspective; Sequence Analysis, DNA; standards and grades
• ISSN: 1087-0156; 1546-1696; 1546-1696
• DOI: 10.1038/nbt.3893

Standards for sequencing the microbial 'uncultivated majority', namely bacterial and archaeal single-cell genome sequences, and genome sequences from metagenomic datasets, are proposed. We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.